High Inductive Magnetic Stimuli and Their Effects on
Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts

J 2019

High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts

PRUCHA, J.; J. SKOPALIK; Ivan JUSTAN; T. PARAK; E. GABRIELOVA et. al.

Basic information

Original name

High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts

Authors

PRUCHA, J.; J. SKOPALIK; Ivan JUSTAN; T. PARAK; E. GABRIELOVA; K. HANA and L. NAVRATIL

Edition

Physiological research, Praha, Fyziologický ústav AV ČR, 2019, 0862-8408

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 1.655

RIV identification code

RIV/00216224:14110/19:00115641

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.33549/physiolres.934382

UT WoS

000529413800009

EID Scopus

2-s2.0-85081131092

Keywords in English

Electromagnetic stimulation; Cell migration; Mesenchymal stromal cells; Fibroblasts; Dendritic cells

Abstract

In the original language

Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m(2). The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO2 incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.

Cite

PRUCHA, J.; J. SKOPALIK; Ivan JUSTAN; T. PARAK; E. GABRIELOVA; K. HANA and L. NAVRATIL. High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts. Physiological research. Praha: Fyziologický ústav AV ČR, 2019, vol. 68, Supplement 4, p. "S433"-"S443", 11 pp. ISSN 0862-8408. Available from: https://doi.org/10.33549/physiolres.934382.

@article{1655477,
   author = {Prucha, J. and Skopalik, J. and Justan, Ivan and Parak, T. and Gabrielova, E. and Hana, K. and Navratil, L.},
   article_location = {Praha},
   article_number = {Supplement 4},
   doi = {https://doi.org/10.33549/physiolres.934382},
   keywords = {Electromagnetic stimulation; Cell migration; Mesenchymal stromal cells; Fibroblasts; Dendritic cells},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological research},
   title = {High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts},
   url = {http://www.biomed.cas.cz/physiolres/pdf/2019/68_S433.pdf},
   volume = {68},
   year = {2019}
}

TY  - JOUR
ID  - 1655477
AU  - Prucha, J. - Skopalik, J. - Justan, Ivan - Parak, T. - Gabrielova, E. - Hana, K. - Navratil, L.
PY  - 2019
TI  - High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts
JF  - Physiological research
VL  - 68
IS  - Supplement 4
SP  - "S433"-"S443"
EP  - "S433"-"S443"
PB  - Fyziologický ústav AV ČR
SN  - 08628408
KW  - Electromagnetic stimulation
KW  - Cell migration
KW  - Mesenchymal stromal cells
KW  - Fibroblasts
KW  - Dendritic cells
UR  - http://www.biomed.cas.cz/physiolres/pdf/2019/68_S433.pdf
L2  - http://www.biomed.cas.cz/physiolres/pdf/2019/68_S433.pdf
N2  - Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m(2). The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO2 incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.
ER  -

PRUCHA, J.; J. SKOPALIK; Ivan JUSTAN; T. PARAK; E. GABRIELOVA; K. HANA and L. NAVRATIL. High Inductive Magnetic Stimuli and Their Effects on Mesenchymal Stromal Cells, Dendritic Cells, and Fibroblasts. \textit{Physiological research}. Praha: Fyziologický ústav AV ČR, 2019, vol.~68, Supplement 4, p.~''S433''-''S443'', 11 pp. ISSN~0862-8408. Available from: https://doi.org/10.33549/physiolres.934382.

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