The evolution of the 9aaTAD domain in Sp2 proteins:
inactivation with valines and intron reservoirs

J 2020

The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs

PISKÁČEK, Martin, Marek HAVELKA, Kristína JENDRUCHOVÁ, Andrea KNIGHT, Liam KEEGAN et. al.

Základní údaje

Originální název

The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs

Autoři

PISKÁČEK, Martin (40 Rakousko, garant, domácí), Marek HAVELKA (203 Česká republika, domácí), Kristína JENDRUCHOVÁ (703 Slovensko, domácí), Andrea KNIGHT (203 Česká republika, domácí) a Liam KEEGAN (372 Irsko, domácí)

Vydání

Cellular and molecular life sciences, BASEL, BIRKHAUSER VERLAG AG, 2020, 1420-682X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.261

Kód RIV

RIV/00216224:14110/20:00118601

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s00018-019-03251-w

UT WoS

000529536500008

Klíčová slova anglicky

KLF; WT1; Gal4; Met4; Gcn4; E2A; MLL; p53; MED15; TAF9; CBP; KIX

Štítky

14110518, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 5. 2020 13:33, Mgr. Tereza Miškechová

Anotace

V originále

The universal nine-amino-acid transactivation domains (9aaTADs) have been identified in numerous transcription activators. Here, we identified the conserved 9aaTAD motif in all nine members of the specificity protein (SP) family. Previously, the Sp1 transcription factor has been defined as a glutamine-rich activator. We showed by amino acid substitutions that the glutamine residues are completely dispensable for 9aaTAD function and are not conserved in the SP family. We described the origin and evolutionary history of 9aaTADs. The 9aaTADs of the ancestral Sp2 gene became inactivated in early chordates. We next discovered that an accumulation of valines in 9aaTADs inactivated their transactivation function and enabled their strict conservation during evolution. Subsequently, in chordates, Sp2 has duplicated and created new paralogs, Sp1, Sp3, and Sp4 (the SP1-4 clade). During chordate evolution, the dormancy of the Sp2 activation domain lasted over 100 million years. The dormant but still intact ancestral Sp2 activation domains allowed diversification of the SP1-4 clade into activators and repressors. By valine substitution in the 9aaTADs, Sp1 and Sp3 regained their original activator function found in ancestral lower metazoan sea sponges. Therefore, the vertebrate SP1-4 clade could include both repressors and activators. Furthermore, we identified secondary 9aaTADs in Sp2 introns present from fish to primates, including humans. In the gibbon genome, introns containing 9aaTADs were used as exons, which turned the Sp2 gene into an activator. Similarly, we identified introns containing 9aaTADs used conditionally as exons in the (SP family-unrelated) transcription factor SREBP1, suggesting that the intron-9aaTAD reservoir is a general phenomenon.

Návaznosti

NV15-32935A, projekt VaV

Název: Analýza gamma-delta T lymfocytů reaktivních na nádorové buňky u pacientů s B-buněčnou chronickou lymfocytární leukémií: nový přístup k buněčné terapii

Citovat

PISKÁČEK, Martin, Marek HAVELKA, Kristína JENDRUCHOVÁ, Andrea KNIGHT a Liam KEEGAN. The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs. Cellular and molecular life sciences. BASEL: BIRKHAUSER VERLAG AG, 2020, roč. 77, č. 9, s. 1793-1810. ISSN 1420-682X. Dostupné z: https://dx.doi.org/10.1007/s00018-019-03251-w.

@article{1656936,
   author = {Piskáček, Martin and Havelka, Marek and Jendruchová, Kristína and Knight, Andrea and Keegan, Liam},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.1007/s00018-019-03251-w},
   keywords = {KLF; WT1; Gal4; Met4; Gcn4; E2A; MLL; p53; MED15; TAF9; CBP; KIX},
   language = {eng},
   issn = {1420-682X},
   journal = {Cellular and molecular life sciences},
   title = {The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs},
   url = {https://link.springer.com/article/10.1007/s00018-019-03251-w},
   volume = {77},
   year = {2020}
}

TY  - JOUR
ID  - 1656936
AU  - Piskáček, Martin - Havelka, Marek - Jendruchová, Kristína - Knight, Andrea - Keegan, Liam
PY  - 2020
TI  - The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs
JF  - Cellular and molecular life sciences
VL  - 77
IS  - 9
SP  - 1793-1810
EP  - 1793-1810
PB  - BIRKHAUSER VERLAG AG
SN  - 1420682X
KW  - KLF
KW  - WT1
KW  - Gal4
KW  - Met4
KW  - Gcn4
KW  - E2A
KW  - MLL
KW  - p53
KW  - MED15
KW  - TAF9
KW  - CBP
KW  - KIX
UR  - https://link.springer.com/article/10.1007/s00018-019-03251-w
L2  - https://link.springer.com/article/10.1007/s00018-019-03251-w
N2  - The universal nine-amino-acid transactivation domains (9aaTADs) have been identified in numerous transcription activators. Here, we identified the conserved 9aaTAD motif in all nine members of the specificity protein (SP) family. Previously, the Sp1 transcription factor has been defined as a glutamine-rich activator. We showed by amino acid substitutions that the glutamine residues are completely dispensable for 9aaTAD function and are not conserved in the SP family. We described the origin and evolutionary history of 9aaTADs. The 9aaTADs of the ancestral Sp2 gene became inactivated in early chordates. We next discovered that an accumulation of valines in 9aaTADs inactivated their transactivation function and enabled their strict conservation during evolution. Subsequently, in chordates, Sp2 has duplicated and created new paralogs, Sp1, Sp3, and Sp4 (the SP1-4 clade). During chordate evolution, the dormancy of the Sp2 activation domain lasted over 100 million years. The dormant but still intact ancestral Sp2 activation domains allowed diversification of the SP1-4 clade into activators and repressors. By valine substitution in the 9aaTADs, Sp1 and Sp3 regained their original activator function found in ancestral lower metazoan sea sponges. Therefore, the vertebrate SP1-4 clade could include both repressors and activators. Furthermore, we identified secondary 9aaTADs in Sp2 introns present from fish to primates, including humans. In the gibbon genome, introns containing 9aaTADs were used as exons, which turned the Sp2 gene into an activator. Similarly, we identified introns containing 9aaTADs used conditionally as exons in the (SP family-unrelated) transcription factor SREBP1, suggesting that the intron-9aaTAD reservoir is a general phenomenon.
ER  -

PISKÁČEK, Martin, Marek HAVELKA, Kristína JENDRUCHOVÁ, Andrea KNIGHT a Liam KEEGAN. The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs. \textit{Cellular and molecular life sciences}. BASEL: BIRKHAUSER VERLAG AG, 2020, roč.~77, č.~9, s.~1793-1810. ISSN~1420-682X. Dostupné z: https://dx.doi.org/10.1007/s00018-019-03251-w.

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