J 2020

Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry

PATEL, Megha, Marek FEITH, Birgit JANICKE, Kersti ALM, Zahra EL-SCHICH et. al.

Základní údaje

Originální název

Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry

Autoři

PATEL, Megha (752 Švédsko), Marek FEITH (203 Česká republika, domácí), Birgit JANICKE (752 Švédsko), Kersti ALM (752 Švédsko) a Zahra EL-SCHICH (752 Švédsko, garant)

Vydání

Applied Sciences, ST ALBAN-ANLAGE, MDPI, 2020, 2076-3417

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10404 Polymer science

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 2.679

Kód RIV

RIV/00216224:14110/20:00115704

Organizační jednotka

Lékařská fakulta

DOI

UT WoS

000525305900021

Klíčová slova anglicky

breast cancer; digital holographic cytometry; molecularly imprinted polymers; motility; sialic acid; viability

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 15. 7. 2020 10:33, Mgr. Tereza Miškechová

Anotace

V originále

Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility.