Bioactive Excreted/Secreted Products of Entomopathogenic
Nematode Heterorhabditis bacteriophora Inhibit the
Phenoloxidase Activity during the Infection

J 2020

Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection

ELIÁŠ, Sara, Jana HURYCHOVÁ, Duarte Nuno TOUBARRO TIAGO, Jorge FRIAS, Martin KUNC et. al.

Základní údaje

Originální název

Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection

Autoři

ELIÁŠ, Sara (276 Německo, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Duarte Nuno TOUBARRO TIAGO (620 Portugalsko), Jorge FRIAS (620 Portugalsko), Martin KUNC (203 Česká republika, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Nelson SIMÕES (620 Portugalsko) a Pavel HYRŠL (203 Česká republika, garant, domácí)

Vydání

Insects, Basel, MDPI, 2020, 2075-4450

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.769

Kód RIV

RIV/00216224:14310/20:00114166

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/insects11060353

UT WoS

000553609400001

Klíčová slova česky

Heterorhabditis bacteriophora; exkretované/sekretované produkty; fenoloxidáza; imunita; melanizace; Galleria mellonella; virulence

Klíčová slova anglicky

Heterorhabditis bacteriophora; excreted/secreted products; phenoloxidase; immunity; melanization; Galleria mellonella; virulence

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 10. 2020 16:39, Mgr. Marie Šípková, DiS.

Anotace

V originále

Entomopathogenic nematodes (EPNs) are efficient insect parasites, that are known for their mutualistic relationship with entomopathogenic bacteria and their use in biocontrol. EPNs produce bioactive molecules referred to as excreted/secreted products (ESPs), which have come to the forefront in recent years because of their role in the process of host invasion and the modulation of its immune response. In the present study, we confirmed the production of ESPs in the EPN Heterorhabditis bacteriophora, and investigated their role in the modulation of the phenoloxidase cascade, one of the key components of the insect immune system. ESPs were isolated from 14- and 21-day-old infective juveniles of H. bacteriophora, which were found to be more virulent than newly emerged nematodes, as was confirmed by mortality assays using Galleria mellonella larvae. The isolated ESPs were further purified and screened for the phenoloxidase-inhibiting activity. In these products, a 38 kDa fraction of peptides was identified as the main candidate source of phenoloxidase-inhibiting compounds. This fraction was further analyzed by mass spectrometry and the de novo sequencing approach. Six peptide sequences were identified in this active ESP fraction, including proteins involved in ubiquitination and the regulation of a Toll pathway, for which a role in the regulation of insect immune response has been proposed in previous studies.

Návaznosti

GA17-03253S, projekt VaV

Název: Hormonální kontrola hmyzího obranného systému

Investor: Grantová agentura ČR, Hormonální kontrola hmyzího obranného systému

Citovat

ELIÁŠ, Sara, Jana HURYCHOVÁ, Duarte Nuno TOUBARRO TIAGO, Jorge FRIAS, Martin KUNC, Pavel DOBEŠ, Nelson SIMÕES a Pavel HYRŠL. Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection. Insects. Basel: MDPI, 2020, roč. 11, č. 6, s. 1-19. ISSN 2075-4450. Dostupné z: https://dx.doi.org/10.3390/insects11060353.

@article{1660778,
   author = {Eliáš, Sara and Hurychová, Jana and Toubarro Tiago, Duarte Nuno and Frias, Jorge and Kunc, Martin and Dobeš, Pavel and Simões, Nelson and Hyršl, Pavel},
   article_location = {Basel},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/insects11060353},
   keywords = {Heterorhabditis bacteriophora; excreted/secreted products; phenoloxidase; immunity; melanization; Galleria mellonella; virulence},
   language = {eng},
   issn = {2075-4450},
   journal = {Insects},
   title = {Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection},
   url = {https://www.mdpi.com/2075-4450/11/6/353},
   volume = {11},
   year = {2020}
}

TY  - JOUR
ID  - 1660778
AU  - Eliáš, Sara - Hurychová, Jana - Toubarro Tiago, Duarte Nuno - Frias, Jorge - Kunc, Martin - Dobeš, Pavel - Simões, Nelson - Hyršl, Pavel
PY  - 2020
TI  - Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection
JF  - Insects
VL  - 11
IS  - 6
SP  - 1-19
EP  - 1-19
PB  - MDPI
SN  - 20754450
KW  - Heterorhabditis bacteriophora
KW  - excreted/secreted products
KW  - phenoloxidase
KW  - immunity
KW  - melanization
KW  - Galleria mellonella
KW  - virulence
UR  - https://www.mdpi.com/2075-4450/11/6/353
L2  - https://www.mdpi.com/2075-4450/11/6/353
N2  - Entomopathogenic nematodes (EPNs) are efficient insect parasites, that are known for their mutualistic relationship with entomopathogenic bacteria and their use in biocontrol. EPNs produce bioactive molecules referred to as excreted/secreted products (ESPs), which have come to the forefront in recent years because of their role in the process of host invasion and the modulation of its immune response. In the present study, we confirmed the production of ESPs in the EPN Heterorhabditis bacteriophora, and investigated their role in the modulation of the phenoloxidase cascade, one of the key components of the insect immune system. ESPs were isolated from 14- and 21-day-old infective juveniles of H. bacteriophora, which were found to be more virulent than newly emerged nematodes, as was confirmed by mortality assays using Galleria mellonella larvae. The isolated ESPs were further purified and screened for the phenoloxidase-inhibiting activity. In these products, a 38 kDa fraction of peptides was identified as the main candidate source of phenoloxidase-inhibiting compounds. This fraction was further analyzed by mass spectrometry and the de novo sequencing approach. Six peptide sequences were identified in this active ESP fraction, including proteins involved in ubiquitination and the regulation of a Toll pathway, for which a role in the regulation of insect immune response has been proposed in previous studies.
ER  -

ELIÁŠ, Sara, Jana HURYCHOVÁ, Duarte Nuno TOUBARRO TIAGO, Jorge FRIAS, Martin KUNC, Pavel DOBEŠ, Nelson SIMÕES a Pavel HYRŠL. Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection. \textit{Insects}. Basel: MDPI, 2020, roč.~11, č.~6, s.~1-19. ISSN~2075-4450. Dostupné z: https://dx.doi.org/10.3390/insects11060353.

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