Cell Proliferation of HaCaT Keratinocytes on Collagen Films
Modified by Argon Plasma Treatment

J 2010

Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment

GARCIA, Jorge Lopez; Ahmad ASADINEZHAD; Jiří PACHERNÍK; Marián LEHOCKÝ; Ita JUNKAR et. al.

Basic information

Original name

Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment

Authors

GARCIA, Jorge Lopez; Ahmad ASADINEZHAD; Jiří PACHERNÍK (203 Czech Republic, belonging to the institution); Marián LEHOCKÝ (203 Czech Republic, guarantor); Ita JUNKAR; Petr HUMPOLÍČEK (203 Czech Republic); Petr SÁHA and Pavel VALASEK

Edition

Blood Cells, Molecules and Diseases, Orlando, Florida, Academic Press, 2010, 1079-9796

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 2.716

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/molecules15042845

UT WoS

000277147400059

EID Scopus

2-s2.0-77951866833

Keywords in English

plasma treatment; atelocollagen; surface modification; HaCaT; cell proliferation

Abstract

In the original language

Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.

Cite

GARCIA, Jorge Lopez; Ahmad ASADINEZHAD; Jiří PACHERNÍK; Marián LEHOCKÝ; Ita JUNKAR; Petr HUMPOLÍČEK; Petr SÁHA and Pavel VALASEK. Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment. Blood Cells, Molecules and Diseases. Orlando, Florida: Academic Press, 2010, vol. 15, No 4, p. 2845-2856. ISSN 1079-9796. Available from: https://dx.doi.org/10.3390/molecules15042845.

@article{1661578,
   author = {Garcia, Jorge Lopez and Asadinezhad, Ahmad and Pacherník, Jiří and Lehocký, Marián and Junkar, Ita and Humpolíček, Petr and Sáha, Petr and Valasek, Pavel},
   article_location = {Orlando, Florida},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/molecules15042845},
   keywords = {plasma treatment; atelocollagen; surface modification; HaCaT; cell proliferation},
   language = {eng},
   issn = {1079-9796},
   journal = {Blood Cells, Molecules and Diseases},
   title = {Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment},
   url = {https://www.mdpi.com/1420-3049/15/4/2845},
   volume = {15},
   year = {2010}
}

TY  - JOUR
ID  - 1661578
AU  - Garcia, Jorge Lopez - Asadinezhad, Ahmad - Pacherník, Jiří - Lehocký, Marián - Junkar, Ita - Humpolíček, Petr - Sáha, Petr - Valasek, Pavel
PY  - 2010
TI  - Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment
JF  - Blood Cells, Molecules and Diseases
VL  - 15
IS  - 4
SP  - 2845-2856
EP  - 2845-2856
PB  - Academic Press
SN  - 10799796
KW  - plasma treatment
KW  - atelocollagen
KW  - surface modification
KW  - HaCaT
KW  - cell proliferation
UR  - https://www.mdpi.com/1420-3049/15/4/2845
L2  - https://www.mdpi.com/1420-3049/15/4/2845
N2  - Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
ER  -

GARCIA, Jorge Lopez; Ahmad ASADINEZHAD; Jiří PACHERNÍK; Marián LEHOCKÝ; Ita JUNKAR; Petr HUMPOLÍČEK; Petr SÁHA and Pavel VALASEK. Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment. \textit{Blood Cells, Molecules and Diseases}. Orlando, Florida: Academic Press, 2010, vol.~15, No~4, p.~2845-2856. ISSN~1079-9796. Available from: https://dx.doi.org/10.3390/molecules15042845.

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