Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA
Polymerase II C-Terminal Domain In Vitro

J 2020

Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro

BRÁZDA, Pavel, Magdaléna KREJČÍKOVÁ, Aiste KASILIAUSKAITE, Eliška ŠMIŘÁKOVÁ, Tomáš KLUMPLER et. al.

Základní údaje

Originální název

Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro

Autoři

BRÁZDA, Pavel (203 Česká republika, domácí), Magdaléna KREJČÍKOVÁ (203 Česká republika, domácí), Aiste KASILIAUSKAITE (440 Litva, domácí), Eliška ŠMIŘÁKOVÁ (203 Česká republika, domácí), Tomáš KLUMPLER (203 Česká republika, domácí), Robert VÁCHA (203 Česká republika, domácí), Karel KUBÍČEK (203 Česká republika, domácí) a Richard ŠTEFL (203 Česká republika, domácí)

Vydání

Journal of Molecular Biology, Academic Press, 2020, 0022-2836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.469

Kód RIV

RIV/00216224:14740/20:00114180

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.jmb.2020.05.007

UT WoS

000543017000010

Klíčová slova anglicky

CTD; RNA polymerase II; Spt6; NMR structure; phosphorylation

Štítky

CF BIC, CF NMR, CF SAXS, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 10. 2024 11:00, Ing. Marie Švancarová

Anotace

V originále

Transcription elongation factor Spt6 associates with RNA polymerase II (RNAP II) via a tandem SH2 (tSH2) domain. The mechanism and significance of the RNAP II-Spt6 interaction is still unclear. Recently, it was proposed that Spt6-tSH2 is recruited via a newly described phosphorylated linker between the Rpb1 core and its C-terminal domain (CTD). Here, we report binding studies with isolated tSH2 of Spt6 (Spt6-tSH2) and Spt6 lacking the first unstructured 297 residues (Spt6N) with a minimal CTD substrate of two repetitive heptads phosphorylated at different sites. The data demonstrate that Spt6 also binds the phosphorylated CTD, a site that was originally proposed as a recognition epitope. We also show that an extended CTD substrate harboring 13 repetitive heptads of the tyrosine-phosphorylated CTD binds Spt6-tSH2 and Spt6N with tighter affinity than the minimal CTD substrate. The enhanced binding is achieved by avidity originating from multiple phosphorylation marks present in the CTD. Interestingly, we found that the steric effects of additional domains in the Spt6N construct partially obscure the binding of the tSH2 domain to the multivalent ligand. We show that Spt6-tSH2 binds various phosphorylation patterns in the CTD and found that the studied combinations of phospho-CTD marks (1,2; 1,5; 2,4; and 2,7) all facilitate the interaction of CTD with Spt6. Our structural studies reveal a plasticity of the tSH2 binding pockets that enables the accommodation of CTDs with phosphorylation marks in different registers.

Návaznosti

GA18-11397S, projekt VaV

Název: Molekulární achitektura komplexů ADAR - RNA

Investor: Grantová agentura ČR, Molecular architecture of ADAR - RNA complexes

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/G/1100/2016, interní kód MU

Název: Computational chemistry for Wnt signaling pathway

Investor: Masarykova univerzita, Computational chemistry for Wnt signaling pathway, INTERDISCIPLINARY - Mezioborové výzkumné projekty

649030, interní kód MU

Název: DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors (Akronym: DECOR)

Investor: Evropská unie, DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors, ERC (Excellent Science)

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

BRÁZDA, Pavel, Magdaléna KREJČÍKOVÁ, Aiste KASILIAUSKAITE, Eliška ŠMIŘÁKOVÁ, Tomáš KLUMPLER, Robert VÁCHA, Karel KUBÍČEK a Richard ŠTEFL. Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro. Journal of Molecular Biology. Academic Press, 2020, roč. 432, č. 14, s. 4092-4107. ISSN 0022-2836. Dostupné z: https://dx.doi.org/10.1016/j.jmb.2020.05.007.

@article{1662603,
   author = {Brázda, Pavel and Krejčíková, Magdaléna and Kasiliauskaite, Aiste and Šmiřáková, Eliška and Klumpler, Tomáš and Vácha, Robert and Kubíček, Karel and Štefl, Richard},
   article_number = {14},
   doi = {http://dx.doi.org/10.1016/j.jmb.2020.05.007},
   keywords = {CTD; RNA polymerase II; Spt6; NMR structure; phosphorylation},
   language = {eng},
   issn = {0022-2836},
   journal = {Journal of Molecular Biology},
   title = {Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro},
   url = {https://www.sciencedirect.com/science/article/pii/S002228362030348X?via%3Dihub},
   volume = {432},
   year = {2020}
}

TY  - JOUR
ID  - 1662603
AU  - Brázda, Pavel - Krejčíková, Magdaléna - Kasiliauskaite, Aiste - Šmiřáková, Eliška - Klumpler, Tomáš - Vácha, Robert - Kubíček, Karel - Štefl, Richard
PY  - 2020
TI  - Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro
JF  - Journal of Molecular Biology
VL  - 432
IS  - 14
SP  - 4092-4107
EP  - 4092-4107
PB  - Academic Press
SN  - 00222836
KW  - CTD
KW  - RNA polymerase II
KW  - Spt6
KW  - NMR structure
KW  - phosphorylation
UR  - https://www.sciencedirect.com/science/article/pii/S002228362030348X?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S002228362030348X?via%3Dihub
N2  - Transcription elongation factor Spt6 associates with RNA polymerase II (RNAP II) via a tandem SH2 (tSH2) domain. The mechanism and significance of the RNAP II-Spt6 interaction is still unclear. Recently, it was proposed that Spt6-tSH2 is recruited via a newly described phosphorylated linker between the Rpb1 core and its C-terminal domain (CTD). Here, we report binding studies with isolated tSH2 of Spt6 (Spt6-tSH2) and Spt6 lacking the first unstructured 297 residues (Spt6N) with a minimal CTD substrate of two repetitive heptads phosphorylated at different sites. The data demonstrate that Spt6 also binds the phosphorylated CTD, a site that was originally proposed as a recognition epitope. We also show that an extended CTD substrate harboring 13 repetitive heptads of the tyrosine-phosphorylated CTD binds Spt6-tSH2 and Spt6N with tighter affinity than the minimal CTD substrate. The enhanced binding is achieved by avidity originating from multiple phosphorylation marks present in the CTD. Interestingly, we found that the steric effects of additional domains in the Spt6N construct partially obscure the binding of the tSH2 domain to the multivalent ligand. We show that Spt6-tSH2 binds various phosphorylation patterns in the CTD and found that the studied combinations of phospho-CTD marks (1,2; 1,5; 2,4; and 2,7) all facilitate the interaction of CTD with Spt6. Our structural studies reveal a plasticity of the tSH2 binding pockets that enables the accommodation of CTDs with phosphorylation marks in different registers.
ER  -

BRÁZDA, Pavel, Magdaléna KREJČÍKOVÁ, Aiste KASILIAUSKAITE, Eliška ŠMIŘÁKOVÁ, Tomáš KLUMPLER, Robert VÁCHA, Karel KUBÍČEK a Richard ŠTEFL. Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro. \textit{Journal of Molecular Biology}. Academic Press, 2020, roč.~432, č.~14, s.~4092-4107. ISSN~0022-2836. Dostupné z: https://dx.doi.org/10.1016/j.jmb.2020.05.007.

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