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@article{1662605,
author = {Han, Z. and Jasnovidova, Olga and Haidara, N. and Tudek, A. and Kubíček, Karel and Libri, D. and Štefl, Richard and Porrua, O.},
article_location = {Hoboken (USA)},
article_number = {7},
doi = {http://dx.doi.org/10.15252/embj.2019101548},
keywords = {non-coding transcription; pervasive transcription; RNA polymerase II CTD; Sen1 helicase; transcription termination},
language = {eng},
issn = {0261-4189},
journal = {EMBO Journal},
title = {Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1},
url = {https://www.embopress.org/doi/full/10.15252/embj.2019101548},
volume = {39},
year = {2020}
}
TY - JOUR
ID - 1662605
AU - Han, Z. - Jasnovidova, Olga - Haidara, N. - Tudek, A. - Kubíček, Karel - Libri, D. - Štefl, Richard - Porrua, O.
PY - 2020
TI - Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1
JF - EMBO Journal
VL - 39
IS - 7
SP - 101548
EP - 101548
PB - WILEY-BLACKWELL
SN - 02614189
KW - non-coding transcription
KW - pervasive transcription
KW - RNA polymerase II CTD
KW - Sen1 helicase
KW - transcription termination
UR - https://www.embopress.org/doi/full/10.15252/embj.2019101548
N2 - Pervasive transcription is a widespread phenomenon leading to the production of a plethora of non-coding RNAs (ncRNAs) without apparent function. Pervasive transcription poses a threat to proper gene expression that needs to be controlled. In yeast, the highly conserved helicase Sen1 restricts pervasive transcription by inducing termination of non-coding transcription. However, the mechanisms underlying the specific function of Sen1 at ncRNAs are poorly understood. Here, we identify a motif in an intrinsically disordered region of Sen1 that mimics the phosphorylated carboxy-terminal domain (CTD) of RNA polymerase II, and structurally characterize its recognition by the CTD-interacting domain of Nrd1, an RNA-binding protein that binds specific sequences in ncRNAs. In addition, we show that Sen1-dependent termination strictly requires CTD recognition by the N-terminal domain of Sen1. We provide evidence that the Sen1-CTD interaction does not promote initial Sen1 recruitment, but rather enhances Sen1 capacity to induce the release of paused RNAPII from the DNA. Our results shed light on the network of protein-protein interactions that control termination of non-coding transcription by Sen1.
ER -
HAN, Z., Olga JASNOVIDOVA, N. HAIDARA, A. TUDEK, Karel KUBÍČEK, D. LIBRI, Richard ŠTEFL a O. PORRUA. Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1. \textit{EMBO Journal}. Hoboken (USA): WILEY-BLACKWELL, 2020, roč.~39, č.~7, s.~101548-101569. ISSN~0261-4189. Dostupné z: https://dx.doi.org/10.15252/embj.2019101548.
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