Termination of non-coding transcription in yeast relies on both
an RNA Pol II CTD interaction domain and a CTD-mimicking region
in Sen1

J 2020

Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1

HAN, Z.; Olga JASNOVIDOVA; N. HAIDARA; A. TUDEK; Karel KUBÍČEK et al.

Základní údaje

Originální název

Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1

Autoři

HAN, Z.; Olga JASNOVIDOVA; N. HAIDARA; A. TUDEK; Karel KUBÍČEK ; D. LIBRI; Richard ŠTEFL a O. PORRUA

Vydání

EMBO Journal, Hoboken (USA), WILEY-BLACKWELL, 2020, 0261-4189

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.598

Kód RIV

RIV/00216224:14740/20:00114181

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.15252/embj.2019101548

UT WoS

000516807800001

EID Scopus

2-s2.0-85080149490

Klíčová slova anglicky

non-coding transcription; pervasive transcription; RNA polymerase II CTD; Sen1 helicase; transcription termination

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2021 18:50, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Pervasive transcription is a widespread phenomenon leading to the production of a plethora of non-coding RNAs (ncRNAs) without apparent function. Pervasive transcription poses a threat to proper gene expression that needs to be controlled. In yeast, the highly conserved helicase Sen1 restricts pervasive transcription by inducing termination of non-coding transcription. However, the mechanisms underlying the specific function of Sen1 at ncRNAs are poorly understood. Here, we identify a motif in an intrinsically disordered region of Sen1 that mimics the phosphorylated carboxy-terminal domain (CTD) of RNA polymerase II, and structurally characterize its recognition by the CTD-interacting domain of Nrd1, an RNA-binding protein that binds specific sequences in ncRNAs. In addition, we show that Sen1-dependent termination strictly requires CTD recognition by the N-terminal domain of Sen1. We provide evidence that the Sen1-CTD interaction does not promote initial Sen1 recruitment, but rather enhances Sen1 capacity to induce the release of paused RNAPII from the DNA. Our results shed light on the network of protein-protein interactions that control termination of non-coding transcription by Sen1.

Návaznosti

GA18-11397S, projekt VaV

Název: Molekulární achitektura komplexů ADAR - RNA

Investor: Grantová agentura ČR, Molecular architecture of ADAR - RNA complexes

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

649030, interní kód MU

Název: DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors (Akronym: DECOR)

Investor: Evropská unie, DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors, ERC (Excellent Science)

Přiložené soubory

embj.2019101548.pdf Verze souboru

Citovat

HAN, Z.; Olga JASNOVIDOVA; N. HAIDARA; A. TUDEK; Karel KUBÍČEK; D. LIBRI; Richard ŠTEFL a O. PORRUA. Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1. EMBO Journal. Hoboken (USA): WILEY-BLACKWELL, 2020, roč. 39, č. 7, s. 101548-101569. ISSN 0261-4189. Dostupné z: https://doi.org/10.15252/embj.2019101548.

@article{1662605,
   author = {Han, Z. and Jasnovidova, Olga and Haidara, N. and Tudek, A. and Kubíček, Karel and Libri, D. and Štefl, Richard and Porrua, O.},
   article_location = {Hoboken (USA)},
   article_number = {7},
   doi = {https://doi.org/10.15252/embj.2019101548},
   keywords = {non-coding transcription; pervasive transcription; RNA polymerase II CTD; Sen1 helicase; transcription termination},
   language = {eng},
   issn = {0261-4189},
   journal = {EMBO Journal},
   title = {Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1},
   url = {https://www.embopress.org/doi/full/10.15252/embj.2019101548},
   volume = {39},
   year = {2020}
}

TY  - JOUR
ID  - 1662605
AU  - Han, Z. - Jasnovidova, Olga - Haidara, N. - Tudek, A. - Kubíček, Karel - Libri, D. - Štefl, Richard - Porrua, O.
PY  - 2020
TI  - Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1
JF  - EMBO Journal
VL  - 39
IS  - 7
SP  - 101548
EP  - 101548
PB  - WILEY-BLACKWELL
SN  - 02614189
KW  - non-coding transcription
KW  - pervasive transcription
KW  - RNA polymerase II CTD
KW  - Sen1 helicase
KW  - transcription termination
UR  - https://www.embopress.org/doi/full/10.15252/embj.2019101548
N2  - Pervasive transcription is a widespread phenomenon leading to the production of a plethora of non-coding RNAs (ncRNAs) without apparent function. Pervasive transcription poses a threat to proper gene expression that needs to be controlled. In yeast, the highly conserved helicase Sen1 restricts pervasive transcription by inducing termination of non-coding transcription. However, the mechanisms underlying the specific function of Sen1 at ncRNAs are poorly understood. Here, we identify a motif in an intrinsically disordered region of Sen1 that mimics the phosphorylated carboxy-terminal domain (CTD) of RNA polymerase II, and structurally characterize its recognition by the CTD-interacting domain of Nrd1, an RNA-binding protein that binds specific sequences in ncRNAs. In addition, we show that Sen1-dependent termination strictly requires CTD recognition by the N-terminal domain of Sen1. We provide evidence that the Sen1-CTD interaction does not promote initial Sen1 recruitment, but rather enhances Sen1 capacity to induce the release of paused RNAPII from the DNA. Our results shed light on the network of protein-protein interactions that control termination of non-coding transcription by Sen1.
ER  -

HAN, Z.; Olga JASNOVIDOVA; N. HAIDARA; A. TUDEK; Karel KUBÍČEK; D. LIBRI; Richard ŠTEFL a O. PORRUA. Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1. \textit{EMBO Journal}. Hoboken (USA): WILEY-BLACKWELL, 2020, roč.~39, č.~7, s.~101548-101569. ISSN~0261-4189. Dostupné z: https://doi.org/10.15252/embj.2019101548.

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