Evaluation of Physiological Parameters of Intestinal
Sulfate-Reducing Bacteria Isolated from Patients Suffering from
IBD and Healthy People

J 2020

Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

KUSHKEVYCH, Ivan, Jorge CASTRO SANGRADOR, Dordević DANI, Monika ROZEHNALOVÁ, Martin ČERNÝ et. al.

Basic information

Original name

Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

Authors

KUSHKEVYCH, Ivan (804 Ukraine, guarantor, belonging to the institution), Jorge CASTRO SANGRADOR (724 Spain, belonging to the institution), Dordević DANI (203 Czech Republic), Monika ROZEHNALOVÁ (203 Czech Republic), Martin ČERNÝ (203 Czech Republic, belonging to the institution), Roman FAFULA, Monika VÍTĚZOVÁ (203 Czech Republic, belonging to the institution) and Simon K.-M. R. RITTMANN (40 Austria)

Edition

Journal of Clinical Medicine, BASEL, MDPI, 2020, 2077-0383

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.241

RIV identification code

RIV/00216224:14310/20:00115827

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/jcm9061920

UT WoS

000549422400001

Keywords (in Czech)

ulcerózní kolitida; střevní mikrobiota, onemocnění střev; redukce sulfátu; sulfid; toxicita

Keywords in English

ulcerative colitis; intestinal microbiota; bowel disease; sulfate reduction; hydrogen sulfide; toxicity

Abstract

V originále

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

Links

MUNI/A/0947/2019, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 4 (Acronym: SV2020)

Investor: Masaryk University, Category A

Citovat

KUSHKEVYCH, Ivan, Jorge CASTRO SANGRADOR, Dordević DANI, Monika ROZEHNALOVÁ, Martin ČERNÝ, Roman FAFULA, Monika VÍTĚZOVÁ and Simon K.-M. R. RITTMANN. Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People. Journal of Clinical Medicine. BASEL: MDPI, 2020, vol. 9, No 6, p. 1-19. ISSN 2077-0383. Available from: https://dx.doi.org/10.3390/jcm9061920.

@article{1663166,
   author = {Kushkevych, Ivan and Castro Sangrador, Jorge and Dani, Dordević and Rozehnalová, Monika and Černý, Martin and Fafula, Roman and Vítězová, Monika and Rittmann, Simon K.andM. R.},
   article_location = {BASEL},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/jcm9061920},
   keywords = {ulcerative colitis; intestinal microbiota; bowel disease; sulfate reduction; hydrogen sulfide; toxicity},
   language = {eng},
   issn = {2077-0383},
   journal = {Journal of Clinical Medicine},
   title = {Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People},
   url = {https://www.mdpi.com/2077-0383/9/6/1920?fbclid=IwAR12Zuxop2zZrngGrGxJEgBUlF1NKBa7fmqx3R6z41KcVPYVA2F3o_kA2wQ},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1663166
AU  - Kushkevych, Ivan - Castro Sangrador, Jorge - Dani, Dordević - Rozehnalová, Monika - Černý, Martin - Fafula, Roman - Vítězová, Monika - Rittmann, Simon K.-M. R.
PY  - 2020
TI  - Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People
JF  - Journal of Clinical Medicine
VL  - 9
IS  - 6
SP  - 1-19
EP  - 1-19
PB  - MDPI
SN  - 20770383
KW  - ulcerative colitis
KW  - intestinal microbiota
KW  - bowel disease
KW  - sulfate reduction
KW  - hydrogen sulfide
KW  - toxicity
UR  - https://www.mdpi.com/2077-0383/9/6/1920?fbclid=IwAR12Zuxop2zZrngGrGxJEgBUlF1NKBa7fmqx3R6z41KcVPYVA2F3o_kA2wQ
L2  - https://www.mdpi.com/2077-0383/9/6/1920?fbclid=IwAR12Zuxop2zZrngGrGxJEgBUlF1NKBa7fmqx3R6z41KcVPYVA2F3o_kA2wQ
N2  - Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.
ER  -

KUSHKEVYCH, Ivan, Jorge CASTRO SANGRADOR, Dordevi$\backslash$'c DANI, Monika ROZEHNALOVÁ, Martin ČERNÝ, Roman FAFULA, Monika VÍTĚZOVÁ and Simon K.-M. R. RITTMANN. Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People. \textit{Journal of Clinical Medicine}. BASEL: MDPI, 2020, vol.~9, No~6, p.~1-19. ISSN~2077-0383. Available from: https://dx.doi.org/10.3390/jcm9061920.

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