Immune-Regulatory Transcriptional Responses in Multiple Organs
of Atlantic Salmon After Tributyltin Exposure, Alone or in
Combination with Forskolin

D 2011

Immune-Regulatory Transcriptional Responses in Multiple Organs of Atlantic Salmon After Tributyltin Exposure, Alone or in Combination with Forskolin

PAVLÍKOVÁ, Nela a Augustine ARUKWE

Základní údaje

Originální název

Immune-Regulatory Transcriptional Responses in Multiple Organs of Atlantic Salmon After Tributyltin Exposure, Alone or in Combination with Forskolin

Autoři

PAVLÍKOVÁ, Nela (203 Česká republika, domácí) a Augustine ARUKWE (garant)

Vydání

PHILADELPHIA, JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, od s. 478-493, 16 s. 2011

Nakladatel

TAYLOR & FRANCIS INC

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10511 Environmental sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Impakt faktor

Impact factor: 1.830

Organizační jednotka

Přírodovědecká fakulta

ISSN

DOI

http://dx.doi.org/10.1080/15287394.2011.550558

UT WoS

000288262000007

EID Scopus

2-s2.0-79952601616

Klíčová slova anglicky

GROWTH-FACTOR-I; TUMOR-NECROSIS-FACTOR; NATURAL-KILLER-CELLS; FINAL OOCYTE MATURATION; DS-NH MICE; GENE-EXPRESSION; VITRO EXPOSURE; COASTAL WATERS; TNF-ALPHA; TGF-BETA

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 6. 2020 13:40, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Tributyltin (TBT) is a widespread marine pollutant that influences physiological conditions of fish and other aquatic organisms. In addition to effects on reproduction, the immune system has been proposed as a possible target for TBT effects. In the present study, the effects of TBT exposure were examined on the expression of genes involved in immune system compentence in liver and head kidney of Atlantic salmon, in the presence and absence of a second-messenger activator (forskolin). Juvenile salmon were force-fed a diet containing TBT (0solvent control, 0.1, 1, or 10 mg/kg fish) for 72 h. Consequently, fish from the control group and 10-mg/kg TBT group were exposed to the adenylate cyclase (AC) activator forskolin (200 g/L) for 2 or 4 h. Forskolin was selected for this study because it is known to exhibit potent immune system enhancement by activating macrophages and lymphocytes. After sacrifice, liver and head kidney were sampled and transcript changes for interleukin (IL)-1, IL-10, transforming growth factor (TGF) , interferon (INF) , INF, tumor necrosis factor (TNF) , Mx3, and insulin-like growth factor (IGF)-1 were determined in both tissues by quantitative polymerase chain reaction (qPCR) using gene-specific primers. TBT, when given alone and also in combination with forskolin, decreased IL-1, TNF, IFN, IFN, Mx3, and IGF-1 gene expression. In contrast, IL-10 and TGF transcripts were increased after TBT exposure alone and also in combination with forskolin. Generally, these effects were largely dependent on TBT dose and time of exposure when given in combination with forskolin. Overall, our findings suggest a possible immunomodulatory effect of TBT, possibly involving cAMP activation.

Citovat

PAVLÍKOVÁ, Nela a Augustine ARUKWE. Immune-Regulatory Transcriptional Responses in Multiple Organs of Atlantic Salmon After Tributyltin Exposure, Alone or in Combination with Forskolin. Online. In JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES. PHILADELPHIA: TAYLOR & FRANCIS INC, 2011, s. 478-493. ISSN 1528-7394. Dostupné z: https://dx.doi.org/10.1080/15287394.2011.550558.

@inproceedings{1664297,
   author = {Pavlíková, Nela and Arukwe, Augustine},
   address = {PHILADELPHIA},
   booktitle = {JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES},
   doi = {http://dx.doi.org/10.1080/15287394.2011.550558},
   keywords = {GROWTH-FACTOR-I; TUMOR-NECROSIS-FACTOR; NATURAL-KILLER-CELLS; FINAL OOCYTE MATURATION; DS-NH MICE; GENE-EXPRESSION; VITRO EXPOSURE; COASTAL WATERS; TNF-ALPHA; TGF-BETA},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {PHILADELPHIA},
   pages = {478-493},
   publisher = {TAYLOR & FRANCIS INC},
   title = {Immune-Regulatory Transcriptional Responses in Multiple Organs of Atlantic Salmon After Tributyltin Exposure, Alone or in Combination with Forskolin},
   url = {https://doi.org/10.1080/15287394.2011.550558},
   year = {2011}
}

TY  - CONF
ID  - 1664297
AU  - Pavlíková, Nela - Arukwe, Augustine
PY  - 2011
TI  - Immune-Regulatory Transcriptional Responses in Multiple Organs of Atlantic Salmon After Tributyltin Exposure, Alone or in Combination with Forskolin
PB  - TAYLOR & FRANCIS INC
CY  - PHILADELPHIA
KW  - GROWTH-FACTOR-I
KW  - TUMOR-NECROSIS-FACTOR
KW  - NATURAL-KILLER-CELLS
KW  - FINAL OOCYTE MATURATION
KW  - DS-NH MICE
KW  - GENE-EXPRESSION
KW  - VITRO EXPOSURE
KW  - COASTAL WATERS
KW  - TNF-ALPHA
KW  - TGF-BETA
UR  - https://doi.org/10.1080/15287394.2011.550558
L2  - https://doi.org/10.1080/15287394.2011.550558
N2  - Tributyltin (TBT) is a widespread marine pollutant that influences physiological conditions of fish and other aquatic organisms. In addition to effects on reproduction, the immune system has been proposed as a possible target for TBT effects. In the present study, the effects of TBT exposure were examined on the expression of genes involved in immune system compentence in liver and head kidney of Atlantic salmon, in the presence and absence of a second-messenger activator (forskolin). Juvenile salmon were force-fed a diet containing TBT (0solvent control, 0.1, 1, or 10 mg/kg fish) for 72 h. Consequently, fish from the control group and 10-mg/kg TBT group were exposed to the adenylate cyclase (AC) activator forskolin (200 g/L) for 2 or 4 h. Forskolin was selected for this study because it is known to exhibit potent immune system enhancement by activating macrophages and lymphocytes. After sacrifice, liver and head kidney were sampled and transcript changes for interleukin (IL)-1, IL-10, transforming growth factor (TGF) , interferon (INF) , INF, tumor necrosis factor (TNF) , Mx3, and insulin-like growth factor (IGF)-1 were determined in both tissues by quantitative polymerase chain reaction (qPCR) using gene-specific primers. TBT, when given alone and also in combination with forskolin, decreased IL-1, TNF, IFN, IFN, Mx3, and IGF-1 gene expression. In contrast, IL-10 and TGF transcripts were increased after TBT exposure alone and also in combination with forskolin. Generally, these effects were largely dependent on TBT dose and time of exposure when given in combination with forskolin. Overall, our findings suggest a possible immunomodulatory effect of TBT, possibly involving cAMP activation.
ER  -

PAVLÍKOVÁ, Nela a Augustine ARUKWE. Immune-Regulatory Transcriptional Responses in Multiple Organs of Atlantic Salmon After Tributyltin Exposure, Alone or in Combination with Forskolin. Online. In \textit{JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES}. PHILADELPHIA: TAYLOR \&{} FRANCIS INC, 2011, s.~478-493. ISSN~1528-7394. Dostupné z: https://dx.doi.org/10.1080/15287394.2011.550558.

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