Identification and age-dependence of pteridines in bed bugs
(Cimex lectularius) and bat bugs (C. pipistrelli) using liquid
chromatography-tandem mass spectrometry

J 2020

Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry

KŘEMENOVÁ, Jana, Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ, Klaus REINHARDT et. al.

Základní údaje

Originální název

Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry

Autoři

KŘEMENOVÁ, Jana (203 Česká republika, garant, domácí), Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ (203 Česká republika, domácí), Klaus REINHARDT, Zdeněk ŠIMEK (203 Česká republika, domácí) a Tomáš BARTONIČKA (203 Česká republika, domácí)

Vydání

Scientific reports, London, Nature Publishing Group, 2020, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.379

Kód RIV

RIV/00216224:14310/20:00114207

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/s41598-020-66919-5

UT WoS

000543993000023

Klíčová slova anglicky

fluorescent pigments; haematophagy; method optimization; insect; age-grading; LC-MS/MS

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 2. 2023 16:14, doc. Mgr. Tomáš Bartonička, Ph.D.

Anotace

V originále

Determining the age of free-living insects, particularly of blood-sucking species, is important for human health because such knowledge critically influences the estimates of biting frequency and vectoring ability. Genetic age determination is currently not available. Pteridines gradually accumulate in the eyes of insects and their concentrations is the prevailing method. Despite of their stability, published extractions differ considerably, including for standards, for mixtures of pteridines and even for light conditions. This methodological inconsistency among studies is likely to influence age estimates severely and to hamper their comparability. Therefore we reviewed methodological steps across 106 studies to identify methodological denominators and results across studies. Second, we experimentally test how different pteridines vary in their age calibration curves in, common bed (Cimex lectularius) and bat bugs (C. pipistrelli). Here we show that the accumulation of particular pteridines varied between a) different populations and b) rearing temperatures but not c) with the impact of light conditions during extraction or d) the type of blood consumed by the bugs. To optimize the extraction of pteridines and measuring concentrations, we recommend the simultaneous measurement of more than one standard and subsequently to select those that show consistent changes over time to differentiate among age cohorts.

Návaznosti

GC18-08468J, projekt VaV

Název: Role adaptace a fenotypové plasticity spermií v ekologické speciaci

Investor: Grantová agentura ČR, Role adaptace a fenotypové plasticity spermií v ekologické speciaci

MUNI/A/1436/2018, interní kód MU

Název: EKologické a EVOluční Principy v populacích obratlovců a jejich parazitů (Akronym: EKEVOP)

Investor: Masarykova univerzita, EKologické a EVOluční Principy v populacích obratlovců a jejich parazitů, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KŘEMENOVÁ, Jana, Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ, Klaus REINHARDT, Zdeněk ŠIMEK a Tomáš BARTONIČKA. Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry. Scientific reports. London: Nature Publishing Group, 2020, roč. 10, č. 1, s. 1-14. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-66919-5.

@article{1664947,
   author = {Křemenová, Jana and Balvín, Ondřej and Otti, Oliver and Pavonič, Michal and Reinhardt, Klaus and Šimek, Zdeněk and Bartonička, Tomáš},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-66919-5},
   keywords = {fluorescent pigments; haematophagy; method optimization; insect; age-grading; LC-MS/MS},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry},
   url = {https://www.nature.com/articles/s41598-020-66919-5#citeas},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1664947
AU  - Křemenová, Jana - Balvín, Ondřej - Otti, Oliver - Pavonič, Michal - Reinhardt, Klaus - Šimek, Zdeněk - Bartonička, Tomáš
PY  - 2020
TI  - Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry
JF  - Scientific reports
VL  - 10
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - Nature Publishing Group
SN  - 20452322
KW  - fluorescent pigments
KW  - haematophagy
KW  - method optimization
KW  - insect
KW  - age-grading
KW  - LC-MS/MS
UR  - https://www.nature.com/articles/s41598-020-66919-5#citeas
L2  - https://www.nature.com/articles/s41598-020-66919-5#citeas
N2  - Determining the age of free-living insects, particularly of blood-sucking species, is important for human health because such knowledge critically influences the estimates of biting frequency and vectoring ability. Genetic age determination is currently not available. Pteridines gradually accumulate in the eyes of insects and their concentrations is the prevailing method. Despite of their stability, published extractions differ considerably, including for standards, for mixtures of pteridines and even for light conditions. This methodological inconsistency among studies is likely to influence age estimates severely and to hamper their comparability. Therefore we reviewed methodological steps across 106 studies to identify methodological denominators and results across studies. Second, we experimentally test how different pteridines vary in their age calibration curves in, common bed (Cimex lectularius) and bat bugs (C. pipistrelli). Here we show that the accumulation of particular pteridines varied between a) different populations and b) rearing temperatures but not c) with the impact of light conditions during extraction or d) the type of blood consumed by the bugs. To optimize the extraction of pteridines and measuring concentrations, we recommend the simultaneous measurement of more than one standard and subsequently to select those that show consistent changes over time to differentiate among age cohorts.
ER  -

KŘEMENOVÁ, Jana, Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ, Klaus REINHARDT, Zdeněk ŠIMEK a Tomáš BARTONIČKA. Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry. \textit{Scientific reports}. London: Nature Publishing Group, 2020, roč.~10, č.~1, s.~1-14. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-66919-5.

Podrobný výpis o publikaci