| LEDVINA, Vojtěch, Karel KLEPÁRNÍK, Soňa LEGARTOVÁ a Eva BÁRTOVÁ. A device for investigation of natural cell mobility and deformability. Electrophoresis. Hoboken: Wiley, 2020, roč. 41, 13-14, s. 1238-1244. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900357. |
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@article{1668300,
author = {Ledvina, Vojtěch and Klepárník, Karel and Legartová, Soňa and Bártová, Eva},
article_location = {Hoboken},
article_number = {13-14},
doi = {http://dx.doi.org/10.1002/elps.201900357},
keywords = {Cell deformability; Cell mobility; Confocal microscopy; Metastasis; Microfluidics},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {A device for investigation of natural cell mobility and deformability},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357},
volume = {41},
year = {2020}
}
TY - JOUR
ID - 1668300
AU - Ledvina, Vojtěch - Klepárník, Karel - Legartová, Soňa - Bártová, Eva
PY - 2020
TI - A device for investigation of natural cell mobility and deformability
JF - Electrophoresis
VL - 41
IS - 13-14
SP - 1238-1244
EP - 1238-1244
PB - Wiley
SN - 01730835
KW - Cell deformability
KW - Cell mobility
KW - Confocal microscopy
KW - Metastasis
KW - Microfluidics
UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357
L2 - https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357
N2 - A microfluidic device made of polydimethylsiloxane was developed for continuous evaluation of natural migration mobility of many eukaryotic cells in relaxed and deformed state. The device was fabricated by standard photolithography and soft lithography techniques using the SU-8 3010 negative photoresist on a glass wafer as the master mold. The simple flow-free device exploits the chemotactic movement of cells through a set of mechanical barriers in the direction of concentration gradients of attractants. The barriers are formed by arrays of circular cross-section pillars with decreasing spacing 7, 5, and 3 mu m. To pass through the obstacles, the cells are deformed and change their cytoskeletal architecture. The instantaneous migration velocities of cells are monitored in a time-lapse setup of the scanning confocal microscope. Thus, the cellular deformability and migratory activity can easily be evaluated. The functionality of the device was tested with model HeLa cells stably transfected with fluorescent Premo FUCCI Cell Cycle Sensor. The designed device has the potential to be implemented for testing the tendency of patients’ tumors to metastasis.
ER -
LEDVINA, Vojtěch, Karel KLEPÁRNÍK, Soňa LEGARTOVÁ a Eva BÁRTOVÁ. A device for investigation of natural cell mobility and deformability. \textit{Electrophoresis}. Hoboken: Wiley, 2020, roč.~41, 13-14, s.~1238-1244. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201900357.
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