Nitrogen nutrition modulates oxidative stress and metabolite
production in Hypericum perforatum

J 2020

Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum

KOVÁČIK, Jozef, Slawomir DRESLER, Viera PETERKOVÁ a Petr BABULA

Základní údaje

Originální název

Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum

Autoři

KOVÁČIK, Jozef (703 Slovensko, garant), Slawomir DRESLER (616 Polsko), Viera PETERKOVÁ (703 Slovensko) a Petr BABULA (203 Česká republika, domácí)

Vydání

Protoplasma, Wien, Springer-Verlag, 2020, 0033-183X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.356

Kód RIV

RIV/00216224:14110/20:00115971

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s00709-019-01448-1

UT WoS

000519379600009

Klíčová slova anglicky

Antioxidants; Fluorescence microscopy; Mineral nutrition; Phenols; Secondary metabolites

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 8. 2020 12:10, Mgr. Tereza Miškechová

Anotace

V originále

Impact of various nitrate concentrations (14.12 mM, 3.53 mM, no nitrate) or ammonium presence (14.12 mM) on physiological and metabolic changes in Hypericum perforatum after 14 days of cultivation was monitored. Nitrate deficiency suppressed growth of shoots but stimulated root growth while ammonium suppressed root growth: concomitant changes of ascorbic acid and glutathione supported these growth changes, e.g., unaltered level in roots under nitrate deficiency but depleted in ammonium treatment. Soluble proteins and water content were more suppressed by nitrate deficiency but total ROS, nitric oxide formation, and antioxidative enzyme activities (APX and SOD) indicate higher sensitivity of plants to ammonium. Though both extreme treatments (NO3- deficiency or ammonium) stimulated accumulation of total soluble phenols and affected PAL activity (in comparison with full or 1/4x nitrate dose), major phenols (chlorogenic acid and three flavonoids) were elevated mainly by NO3- deficiency. At the level of specific metabolites, NO3- deficiency had stimulatory impact on pseudohypericin (but not hypericin) content while hyperforin decreased. Expression of earlier putative gene of hypericin biosynthesis (hyp-1) showed rather partial correlation with pseudohypericin amount. Data indicate that depletion of NO3- is useful to obtain Hypericum plants with higher amount of health-positive secondary metabolites.

Citovat

KOVÁČIK, Jozef, Slawomir DRESLER, Viera PETERKOVÁ a Petr BABULA. Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum. Protoplasma. Wien: Springer-Verlag, 2020, roč. 257, č. 2, s. 439-447. ISSN 0033-183X. Dostupné z: https://dx.doi.org/10.1007/s00709-019-01448-1.

@article{1670235,
   author = {Kováčik, Jozef and Dresler, Slawomir and Peterková, Viera and Babula, Petr},
   article_location = {Wien},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s00709-019-01448-1},
   keywords = {Antioxidants; Fluorescence microscopy; Mineral nutrition; Phenols; Secondary metabolites},
   language = {eng},
   issn = {0033-183X},
   journal = {Protoplasma},
   title = {Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum},
   url = {https://link.springer.com/article/10.1007/s00709-019-01448-1},
   volume = {257},
   year = {2020}
}

TY  - JOUR
ID  - 1670235
AU  - Kováčik, Jozef - Dresler, Slawomir - Peterková, Viera - Babula, Petr
PY  - 2020
TI  - Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum
JF  - Protoplasma
VL  - 257
IS  - 2
SP  - 439-447
EP  - 439-447
PB  - Springer-Verlag
SN  - 0033183X
KW  - Antioxidants
KW  - Fluorescence microscopy
KW  - Mineral nutrition
KW  - Phenols
KW  - Secondary metabolites
UR  - https://link.springer.com/article/10.1007/s00709-019-01448-1
L2  - https://link.springer.com/article/10.1007/s00709-019-01448-1
N2  - Impact of various nitrate concentrations (14.12 mM, 3.53 mM, no nitrate) or ammonium presence (14.12 mM) on physiological and metabolic changes in Hypericum perforatum after 14 days of cultivation was monitored. Nitrate deficiency suppressed growth of shoots but stimulated root growth while ammonium suppressed root growth: concomitant changes of ascorbic acid and glutathione supported these growth changes, e.g., unaltered level in roots under nitrate deficiency but depleted in ammonium treatment. Soluble proteins and water content were more suppressed by nitrate deficiency but total ROS, nitric oxide formation, and antioxidative enzyme activities (APX and SOD) indicate higher sensitivity of plants to ammonium. Though both extreme treatments (NO3- deficiency or ammonium) stimulated accumulation of total soluble phenols and affected PAL activity (in comparison with full or 1/4x nitrate dose), major phenols (chlorogenic acid and three flavonoids) were elevated mainly by NO3- deficiency. At the level of specific metabolites, NO3- deficiency had stimulatory impact on pseudohypericin (but not hypericin) content while hyperforin decreased. Expression of earlier putative gene of hypericin biosynthesis (hyp-1) showed rather partial correlation with pseudohypericin amount. Data indicate that depletion of NO3- is useful to obtain Hypericum plants with higher amount of health-positive secondary metabolites.
ER  -

KOVÁČIK, Jozef, Slawomir DRESLER, Viera PETERKOVÁ a Petr BABULA. Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum. \textit{Protoplasma}. Wien: Springer-Verlag, 2020, roč.~257, č.~2, s.~439-447. ISSN~0033-183X. Dostupné z: https://dx.doi.org/10.1007/s00709-019-01448-1.

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