Identification of Aspergillus Conidia in Bronchoalveolar Lavage
Using Offline Combination of Capillary Electrophoresis in
Supercritical Water-Treated Fused Silica Capillary and
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass
Spectrometry

J 2020

Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA, Michal ROTH et. al.

Základní údaje

Originální název

Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Autoři

SALPLACHTA, Jiri (203 Česká republika, garant), Marie HORKA (203 Česká republika), Pavel KARASEK (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, domácí) a Michal ROTH (203 Česká republika)

Vydání

Analytical chemistry, WASHINGTON, AMER CHEMICAL SOC, 2020, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.986

Kód RIV

RIV/00216224:14110/20:00114808

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1021/acs.analchem.0c00165

UT WoS

000538417400032

Klíčová slova anglicky

MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 7. 2020 09:29, Mgr. Tereza Miškechová

Anotace

V originále

Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.

Návaznosti

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, projekt VaV

Název: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Akronym: VMIMRDIBT)

Investor: Ministerstvo vnitra ČR, Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů

Citovat

SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA a Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Analytical chemistry. WASHINGTON: AMER CHEMICAL SOC, 2020, roč. 92, č. 11, s. 7588-7595. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.0c00165.

@article{1670470,
   author = {Salplachta, Jiri and Horka, Marie and Karasek, Pavel and Růžička, Filip and Roth, Michal},
   article_location = {WASHINGTON},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.analchem.0c00165},
   keywords = {MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#},
   volume = {92},
   year = {2020}
}

TY  - JOUR
ID  - 1670470
AU  - Salplachta, Jiri - Horka, Marie - Karasek, Pavel - Růžička, Filip - Roth, Michal
PY  - 2020
TI  - Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
JF  - Analytical chemistry
VL  - 92
IS  - 11
SP  - 7588-7595
EP  - 7588-7595
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - MALDI-TOF MS
KW  - SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS
KW  - DIFFERENT INTERNAL DIAMETERS
KW  - INVASIVE FUNGAL-INFECTIONS
KW  - DESORPTION IONIZATION-TIME
KW  - METHICILLIN-RESISTANT
KW  - DIAGNOSTIC METHODS
KW  - NEUTRAL ANALYTES
KW  - 2 SEGMENTS
KW  - MICROORGANISMS
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
L2  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
N2  - Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.
ER  -

SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA a Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. \textit{Analytical chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2020, roč.~92, č.~11, s.~7588-7595. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.0c00165.

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