Identification of Aspergillus Conidia in Bronchoalveolar Lavage
Using Offline Combination of Capillary Electrophoresis in
Supercritical Water-Treated Fused Silica Capillary and
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass
Spectrometry

J 2020

Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

SALPLACHTA, Jiri; Marie HORKA; Pavel KARASEK; Filip RŮŽIČKA; Michal ROTH et. al.

Basic information

Original name

Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Authors

SALPLACHTA, Jiri (203 Czech Republic, guarantor); Marie HORKA (203 Czech Republic); Pavel KARASEK (203 Czech Republic); Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Michal ROTH (203 Czech Republic)

Edition

Analytical chemistry, WASHINGTON, AMER CHEMICAL SOC, 2020, 0003-2700

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 6.986

RIV identification code

RIV/00216224:14110/20:00114808

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1021/acs.analchem.0c00165

UT WoS

000538417400032

EID Scopus

2-s2.0-85085903487

Keywords in English

MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS

Abstract

In the original language

Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.

Links

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, research and development project

Name: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Acronym: VMIMRDIBT)

Investor: Ministry of the Interior of the CR

Cite

SALPLACHTA, Jiri; Marie HORKA; Pavel KARASEK; Filip RŮŽIČKA and Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Analytical chemistry. WASHINGTON: AMER CHEMICAL SOC, 2020, vol. 92, No 11, p. 7588-7595. ISSN 0003-2700. Available from: https://doi.org/10.1021/acs.analchem.0c00165.

@article{1670470,
   author = {Salplachta, Jiri and Horka, Marie and Karasek, Pavel and Růžička, Filip and Roth, Michal},
   article_location = {WASHINGTON},
   article_number = {11},
   doi = {https://doi.org/10.1021/acs.analchem.0c00165},
   keywords = {MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#},
   volume = {92},
   year = {2020}
}

TY  - JOUR
ID  - 1670470
AU  - Salplachta, Jiri - Horka, Marie - Karasek, Pavel - Růžička, Filip - Roth, Michal
PY  - 2020
TI  - Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
JF  - Analytical chemistry
VL  - 92
IS  - 11
SP  - 7588-7595
EP  - 7588-7595
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - MALDI-TOF MS
KW  - SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS
KW  - DIFFERENT INTERNAL DIAMETERS
KW  - INVASIVE FUNGAL-INFECTIONS
KW  - DESORPTION IONIZATION-TIME
KW  - METHICILLIN-RESISTANT
KW  - DIAGNOSTIC METHODS
KW  - NEUTRAL ANALYTES
KW  - 2 SEGMENTS
KW  - MICROORGANISMS
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
L2  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
N2  - Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.
ER  -

SALPLACHTA, Jiri; Marie HORKA; Pavel KARASEK; Filip RŮŽIČKA and Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. \textit{Analytical chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2020, vol.~92, No~11, p.~7588-7595. ISSN~0003-2700. Available from: https://doi.org/10.1021/acs.analchem.0c00165.

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