Whole genome sequence of the Treponema pallidum subsp.
endemicum strain Iraq B: A subpopulation of bejel treponemes
contains full-length tprF and tprG genes similar to those
present in T. p. subsp. pertenue strains

J 2020

Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains

MIKALOVÁ, Lenka, Klára JANEČKOVÁ, Markéta NOVÁKOVÁ, Michal STROUHAL, Darina ČEJKOVÁ et. al.

Základní údaje

Originální název

Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains

Autoři

MIKALOVÁ, Lenka (203 Česká republika, domácí), Klára JANEČKOVÁ (203 Česká republika, domácí), Markéta NOVÁKOVÁ (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Darina ČEJKOVÁ (203 Česká republika), K. N. HARPER (840 Spojené státy) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

Plos One, San Francisco, Public Library Science, 2020, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.240

Kód RIV

RIV/00216224:14110/20:00114235

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0230926

UT WoS

000535938700042

Klíčová slova anglicky

ANTIGENIC VARIATION; SYPHILIS; DIVERSITY; REGIONS; NICHOLS; YAWS; CELL

Štítky

14110513

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 2. 2021 14:39, Mgr. Tereza Miškechová

Anotace

V originále

Treponema pallidum subsp. endemicum (TEN) is the causative agent of endemic syphilis (bejel). Until now, only a single TEN strain, Bosnia A, has been completely sequenced. The only other laboratory TEN strain available, Iraq B, was isolated in Iraq in 1951 by researchers from the US Centers for Disease Control and Prevention. In this study, the complete genome of the Iraq B strain was amplified as overlapping PCR products and sequenced using the pooled segment genome sequencing method and Illumina sequencing. Total average genome sequencing coverage reached 3469x, with a total genome size of 1,137,653 bp. Compared to the genome sequence of Bosnia A, a set of 37 single nucleotide differences, 4 indels, 2 differences in the number of tandem repetitions, and 18 differences in the length of homopolymeric regions were found in the Iraq B genome. Moreover, the tprF and tprG genes that were previously found deleted in the genome of the TEN Bosnia A strain (spanning 2.3 kb in length) were present in a subpopulation of TEN Iraq B and Bosnia A microbes, and their sequence was highly similar to those found in T. p. subsp. pertenue strains, which cause the disease yaws. The genome sequence of TEN Iraq B revealed close genetic relatedness between both available bejel-causing laboratory strains (i.e., Iraq B and Bosnia A) and also genetic variability within the bejel treponemes comparable to that found within yaws- or syphilis-causing strains. In addition, genetic relatedness to TPE strains was demonstrated by the sequence of the tprF and tprG genes found in sub-populations of both TEN Iraq B and Bosnia A. The loss of the tprF and tprG genes in most TEN microbes suggest that TEN genomes have been evolving via the loss of genomic regions, a phenomenon previously found among the treponemes causing both syphilis and rabbit syphilis.

Návaznosti

GA17-25455S, projekt VaV

Název: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Grantová agentura ČR, Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

GC18-23521J, projekt VaV

Název: Treponematózy u zástupců řádu zajícovci: genetická diverzita treponem a příbuznost s lidským patogenem T. pallidum

Investor: Grantová agentura ČR, Treponematózy u zástupců řádu zajícovci: genetická diverzita treponem a příbuznost s lidským patogenem T. pallidum

GJ17-25589Y, projekt VaV

Název: Kmeny způsobující syfilis: jsou geneticky odlišné kmeny odlišné i v průběhu experimentální infekce?

Investor: Grantová agentura ČR, Genetically distinct groups of syphilis-causing strains: do they differ during experimental infection?

Citovat

MIKALOVÁ, Lenka, Klára JANEČKOVÁ, Markéta NOVÁKOVÁ, Michal STROUHAL, Darina ČEJKOVÁ, K. N. HARPER a David ŠMAJS. Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains. Plos One. San Francisco: Public Library Science, 2020, roč. 15, č. 4, s. 1-16. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0230926.

@article{1670600,
   author = {Mikalová, Lenka and Janečková, Klára and Nováková, Markéta and Strouhal, Michal and Čejková, Darina and Harper, K. N. and Šmajs, David},
   article_location = {San Francisco},
   article_number = {4},
   doi = {http://dx.doi.org/10.1371/journal.pone.0230926},
   keywords = {ANTIGENIC VARIATION; SYPHILIS; DIVERSITY; REGIONS; NICHOLS; YAWS; CELL},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos One},
   title = {Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains},
   url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0230926&type=printable},
   volume = {15},
   year = {2020}
}

TY  - JOUR
ID  - 1670600
AU  - Mikalová, Lenka - Janečková, Klára - Nováková, Markéta - Strouhal, Michal - Čejková, Darina - Harper, K. N. - Šmajs, David
PY  - 2020
TI  - Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains
JF  - Plos One
VL  - 15
IS  - 4
SP  - 1-16
EP  - 1-16
PB  - Public Library Science
SN  - 19326203
KW  - ANTIGENIC VARIATION
KW  - SYPHILIS
KW  - DIVERSITY
KW  - REGIONS
KW  - NICHOLS
KW  - YAWS
KW  - CELL
UR  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0230926&type=printable
L2  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0230926&type=printable
N2  - Treponema pallidum subsp. endemicum (TEN) is the causative agent of endemic syphilis (bejel). Until now, only a single TEN strain, Bosnia A, has been completely sequenced. The only other laboratory TEN strain available, Iraq B, was isolated in Iraq in 1951 by researchers from the US Centers for Disease Control and Prevention. In this study, the complete genome of the Iraq B strain was amplified as overlapping PCR products and sequenced using the pooled segment genome sequencing method and Illumina sequencing. Total average genome sequencing coverage reached 3469x, with a total genome size of 1,137,653 bp. Compared to the genome sequence of Bosnia A, a set of 37 single nucleotide differences, 4 indels, 2 differences in the number of tandem repetitions, and 18 differences in the length of homopolymeric regions were found in the Iraq B genome. Moreover, the tprF and tprG genes that were previously found deleted in the genome of the TEN Bosnia A strain (spanning 2.3 kb in length) were present in a subpopulation of TEN Iraq B and Bosnia A microbes, and their sequence was highly similar to those found in T. p. subsp. pertenue strains, which cause the disease yaws. The genome sequence of TEN Iraq B revealed close genetic relatedness between both available bejel-causing laboratory strains (i.e., Iraq B and Bosnia A) and also genetic variability within the bejel treponemes comparable to that found within yaws- or syphilis-causing strains. In addition, genetic relatedness to TPE strains was demonstrated by the sequence of the tprF and tprG genes found in sub-populations of both TEN Iraq B and Bosnia A. The loss of the tprF and tprG genes in most TEN microbes suggest that TEN genomes have been evolving via the loss of genomic regions, a phenomenon previously found among the treponemes causing both syphilis and rabbit syphilis.
ER  -

MIKALOVÁ, Lenka, Klára JANEČKOVÁ, Markéta NOVÁKOVÁ, Michal STROUHAL, Darina ČEJKOVÁ, K. N. HARPER a David ŠMAJS. Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains. \textit{Plos One}. San Francisco: Public Library Science, 2020, roč.~15, č.~4, s.~1-16. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0230926.

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