Long-term impact of cadmium in protonema cultures of
Physcomitrella patens

J 2020

Long-term impact of cadmium in protonema cultures of Physcomitrella patens

KOVACIK, Jozef, Slawomir DRESLER a Petr BABULA

Základní údaje

Originální název

Long-term impact of cadmium in protonema cultures of Physcomitrella patens

Autoři

KOVACIK, Jozef (703 Slovensko, garant), Slawomir DRESLER (616 Polsko) a Petr BABULA (203 Česká republika, domácí)

Vydání

Ecotoxicology and Environmental Safety, San Diego, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020, 0147-6513

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Slovinsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.291

Kód RIV

RIV/00216224:14110/20:00116022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.ecoenv.2020.110333

UT WoS

000521519900002

Klíčová slova anglicky

Antioxidative enzymes; Fluorescence microscopy; Heavy metals; Oxidative stress

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 8. 2020 10:33, Mgr. Tereza Miškechová

Anotace

V originále

Antioxidative responses of axenic protonema cultures of the moss Physcomitrella patens exposed to 10 mu M Cd over 40 d were studied. Cd treatment suppressed growth by ca. 75% with concomitant browning of some filaments and suppression of chlorophyll autofluorescence but had no impact on tissue water content. Despite this negative growth responses which could be related to enhanced ROS formation (as detected using fluorescence staining reagents for total ROS, hydroperoxides and lipid peroxidation), some metabolites revealed strong elevation by Cd which could contribute to attenuation of long-term Cd stress (elevation of ascorbic, malic and citric acids). Molar ratio of malate to Cd was 12.7 and citrate to Cd 2.5, thus potentially contributing to Cd chelation. Interestingly, GSH/GSSG pool and nitric oxide formation remained unaltered by Cd. Accumulation of Cd reached 82 mu g/g DW with bioaccumulation factor of 73. Data indicate that Cd induces elevation of potentially protective metabolites even after prolonged exposure though they do not prevent oxidative stress sufficiently.

Citovat

KOVACIK, Jozef, Slawomir DRESLER a Petr BABULA. Long-term impact of cadmium in protonema cultures of Physcomitrella patens. Ecotoxicology and Environmental Safety. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020, roč. 193, April 2020, s. 1-6. ISSN 0147-6513. Dostupné z: https://dx.doi.org/10.1016/j.ecoenv.2020.110333.

@article{1670604,
   author = {Kovacik, Jozef and Dresler, Slawomir and Babula, Petr},
   article_location = {San Diego},
   article_number = {April 2020},
   doi = {http://dx.doi.org/10.1016/j.ecoenv.2020.110333},
   keywords = {Antioxidative enzymes; Fluorescence microscopy; Heavy metals; Oxidative stress},
   language = {eng},
   issn = {0147-6513},
   journal = {Ecotoxicology and Environmental Safety},
   title = {Long-term impact of cadmium in protonema cultures of Physcomitrella patens},
   url = {https://www.sciencedirect.com/science/article/pii/S014765132030172X?via%3Dihub},
   volume = {193},
   year = {2020}
}

TY  - JOUR
ID  - 1670604
AU  - Kovacik, Jozef - Dresler, Slawomir - Babula, Petr
PY  - 2020
TI  - Long-term impact of cadmium in protonema cultures of Physcomitrella patens
JF  - Ecotoxicology and Environmental Safety
VL  - 193
IS  - April 2020
SP  - 1-6
EP  - 1-6
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 01476513
KW  - Antioxidative enzymes
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
UR  - https://www.sciencedirect.com/science/article/pii/S014765132030172X?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S014765132030172X?via%3Dihub
N2  - Antioxidative responses of axenic protonema cultures of the moss Physcomitrella patens exposed to 10 mu M Cd over 40 d were studied. Cd treatment suppressed growth by ca. 75% with concomitant browning of some filaments and suppression of chlorophyll autofluorescence but had no impact on tissue water content. Despite this negative growth responses which could be related to enhanced ROS formation (as detected using fluorescence staining reagents for total ROS, hydroperoxides and lipid peroxidation), some metabolites revealed strong elevation by Cd which could contribute to attenuation of long-term Cd stress (elevation of ascorbic, malic and citric acids). Molar ratio of malate to Cd was 12.7 and citrate to Cd 2.5, thus potentially contributing to Cd chelation. Interestingly, GSH/GSSG pool and nitric oxide formation remained unaltered by Cd. Accumulation of Cd reached 82 mu g/g DW with bioaccumulation factor of 73. Data indicate that Cd induces elevation of potentially protective metabolites even after prolonged exposure though they do not prevent oxidative stress sufficiently.
ER  -

KOVACIK, Jozef, Slawomir DRESLER a Petr BABULA. Long-term impact of cadmium in protonema cultures of Physcomitrella patens. \textit{Ecotoxicology and Environmental Safety}. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020, roč.~193, April 2020, s.~1-6. ISSN~0147-6513. Dostupné z: https://dx.doi.org/10.1016/j.ecoenv.2020.110333.

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