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@article{1670721,
author = {Dadáková, Kateřina and Trnková, Andrea and Kašparovská, Jitka and Křížová, Ludmila and Lochman, Jan and Kašparovský, Tomáš},
article_location = {Hoboken},
article_number = {6},
doi = {http://dx.doi.org/10.1111/jpn.13402},
keywords = {diet; equol; Isoflavone; metabolism; rumen fluid},
language = {eng},
issn = {0931-2439},
journal = {Journal of Animal Physiology and Animal Nutrition},
title = {In vitro metabolism of red clover isoflavones in rumen fluid},
url = {https://onlinelibrary.wiley.com/doi/10.1111/jpn.13402},
volume = {104},
year = {2020}
}
TY - JOUR
ID - 1670721
AU - Dadáková, Kateřina - Trnková, Andrea - Kašparovská, Jitka - Křížová, Ludmila - Lochman, Jan - Kašparovský, Tomáš
PY - 2020
TI - In vitro metabolism of red clover isoflavones in rumen fluid
JF - Journal of Animal Physiology and Animal Nutrition
VL - 104
IS - 6
SP - 1647-1654
EP - 1647-1654
PB - Wiley
SN - 09312439
KW - diet
KW - equol
KW - Isoflavone
KW - metabolism
KW - rumen fluid
UR - https://onlinelibrary.wiley.com/doi/10.1111/jpn.13402
L2 - https://onlinelibrary.wiley.com/doi/10.1111/jpn.13402
N2 - The degradation of red clover isoflavones was studied in vitro using a rumen fluid buffer system. Various amounts of red clover extract (5-75 mg) together with hay or concentrate-rich diet were added to 40 ml of rumen fluid obtained from non-lactating and lactating dairy cows, respectively, and incubated for 0, 3, 6, 12 or 24 hr. Following incubation, concentrations of daidzein, genistein, formononetin, biochanin A and equol were determined in the samples. After 3 hr of incubation, isoflavone metabolism and equol production could be observed. The results obtained indicate that hay diet provides better conditions for isoflavone metabolism, as concentrations of daidzein, formononetin and biochanin A were higher in incubations based on the concentrate-rich diet and the production of equol was higher in incubations based on the hay diet. Furthermore, in incubations with higher amounts of added clover extract, a decrease in equol production was observed. Further studies are needed to clarify the role of adaptation of rumen microflora on isoflavone degradation kinetics and to clarify the interrelationship between various dietary factors, rumen microbiota and isoflavones. The knowledge of isoflavone metabolism kinetics in dependence on studied factors will be useful for the optimization of feeding dose.
ER -
DADÁKOVÁ, Kateřina, Andrea TRNKOVÁ, Jitka KAŠPAROVSKÁ, Ludmila KŘÍŽOVÁ, Jan LOCHMAN and Tomáš KAŠPAROVSKÝ. In vitro metabolism of red clover isoflavones in rumen fluid. \textit{Journal of Animal Physiology and Animal Nutrition}. Hoboken: Wiley, 2020, vol.~104, No~6, p.~1647-1654. ISSN~0931-2439. Available from: https://dx.doi.org/10.1111/jpn.13402.
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