Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus
spp. recognize O-methylated sugars and influence the host
immune system

J 2021

Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system

FUJDIAROVÁ, Eva, Josef HOUSER, Pavel DOBEŠ, Gita PAULÍKOVÁ, Nikolaj KONDAKOV et. al.

Základní údaje

Originální název

Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system

Autoři

FUJDIAROVÁ, Eva (203 Česká republika, domácí), Josef HOUSER (203 Česká republika, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Gita PAULÍKOVÁ (203 Česká republika, domácí), Nikolaj KONDAKOV, Leonid KONONOV, Pavel HYRŠL (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

The FEBS Journal, Hoboken, Wiley, 2021, 1742-464X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.622

Kód RIV

RIV/00216224:14310/21:00118748

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1111/febs.15457

UT WoS

000545950100001

Klíčová slova česky

lektin; O-methylace; fenoloxidáza; Photorhabdus; reaktivní formy kyslíku

Klíčová slova anglicky

lectin; O-methylation; phenoloxidase; Photorhabdus; reactive oxygen species

Štítky

CF BIC, CF CELLIM, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 09:10, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

O-methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen-associated molecular pattern. We describe the interaction of O-methylated saccharides with two beta-propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica. The crystal structures of PLL2 and PHL revealed up to 10 out of 14 potential binding sites per protein subunit to be occupied with O-methylated structures. The avidity effect strengthens the interaction by 4 orders of magnitude. PLL2 and PHL also interfere with the early immune response by modulating the production of reactive oxygen species and phenoloxidase activity. Since bacteria from Photorhabdus spp. have a complex life cycle involving pathogenicity towards different hosts, the involvement of PLL2 and PHL might contribute to the pathogen overcoming insect and human immune system defenses in the early stages of infection.

Návaznosti

GA18-18964S, projekt VaV

Název: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Grantová agentura ČR, Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

FUJDIAROVÁ, Eva, Josef HOUSER, Pavel DOBEŠ, Gita PAULÍKOVÁ, Nikolaj KONDAKOV, Leonid KONONOV, Pavel HYRŠL a Michaela WIMMEROVÁ. Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system. The FEBS Journal. Hoboken: Wiley, 2021, roč. 288, č. 4, s. 1343-1365. ISSN 1742-464X. Dostupné z: https://dx.doi.org/10.1111/febs.15457.

@article{1671296,
   author = {Fujdiarová, Eva and Houser, Josef and Dobeš, Pavel and Paulíková, Gita and Kondakov, Nikolaj and Kononov, Leonid and Hyršl, Pavel and Wimmerová, Michaela},
   article_location = {Hoboken},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/febs.15457},
   keywords = {lectin; O-methylation; phenoloxidase; Photorhabdus; reactive oxygen species},
   language = {eng},
   issn = {1742-464X},
   journal = {The FEBS Journal},
   title = {Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system},
   url = {https://doi.org/10.1111/febs.15457},
   volume = {288},
   year = {2021}
}

TY  - JOUR
ID  - 1671296
AU  - Fujdiarová, Eva - Houser, Josef - Dobeš, Pavel - Paulíková, Gita - Kondakov, Nikolaj - Kononov, Leonid - Hyršl, Pavel - Wimmerová, Michaela
PY  - 2021
TI  - Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system
JF  - The FEBS Journal
VL  - 288
IS  - 4
SP  - 1343-1365
EP  - 1343-1365
PB  - Wiley
SN  - 1742464X
KW  - lectin
KW  - O-methylation
KW  - phenoloxidase
KW  - Photorhabdus
KW  - reactive oxygen species
UR  - https://doi.org/10.1111/febs.15457
L2  - https://doi.org/10.1111/febs.15457
N2  - O-methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen-associated molecular pattern. We describe the interaction of O-methylated saccharides with two beta-propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica. The crystal structures of PLL2 and PHL revealed up to 10 out of 14 potential binding sites per protein subunit to be occupied with O-methylated structures. The avidity effect strengthens the interaction by 4 orders of magnitude. PLL2 and PHL also interfere with the early immune response by modulating the production of reactive oxygen species and phenoloxidase activity. Since bacteria from Photorhabdus spp. have a complex life cycle involving pathogenicity towards different hosts, the involvement of PLL2 and PHL might contribute to the pathogen overcoming insect and human immune system defenses in the early stages of infection.
ER  -

FUJDIAROVÁ, Eva, Josef HOUSER, Pavel DOBEŠ, Gita PAULÍKOVÁ, Nikolaj KONDAKOV, Leonid KONONOV, Pavel HYRŠL a Michaela WIMMEROVÁ. Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system. \textit{The FEBS Journal}. Hoboken: Wiley, 2021, roč.~288, č.~4, s.~1343-1365. ISSN~1742-464X. Dostupné z: https://dx.doi.org/10.1111/febs.15457.

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