| FUJDIAROVÁ, Eva, Josef HOUSER, Pavel DOBEŠ, Gita PAULÍKOVÁ, Nikolaj KONDAKOV, Leonid KONONOV, Pavel HYRŠL a Michaela WIMMEROVÁ. Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system. The FEBS Journal. Hoboken: Wiley, 2021, roč. 288, č. 4, s. 1343-1365. ISSN 1742-464X. Dostupné z: https://dx.doi.org/10.1111/febs.15457. |
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@article{1671296,
author = {Fujdiarová, Eva and Houser, Josef and Dobeš, Pavel and Paulíková, Gita and Kondakov, Nikolaj and Kononov, Leonid and Hyršl, Pavel and Wimmerová, Michaela},
article_location = {Hoboken},
article_number = {4},
doi = {http://dx.doi.org/10.1111/febs.15457},
keywords = {lectin; O-methylation; phenoloxidase; Photorhabdus; reactive oxygen species},
language = {eng},
issn = {1742-464X},
journal = {The FEBS Journal},
title = {Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system},
url = {https://doi.org/10.1111/febs.15457},
volume = {288},
year = {2021}
}
TY - JOUR
ID - 1671296
AU - Fujdiarová, Eva - Houser, Josef - Dobeš, Pavel - Paulíková, Gita - Kondakov, Nikolaj - Kononov, Leonid - Hyršl, Pavel - Wimmerová, Michaela
PY - 2021
TI - Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system
JF - The FEBS Journal
VL - 288
IS - 4
SP - 1343-1365
EP - 1343-1365
PB - Wiley
SN - 1742464X
KW - lectin
KW - O-methylation
KW - phenoloxidase
KW - Photorhabdus
KW - reactive oxygen species
UR - https://doi.org/10.1111/febs.15457
L2 - https://doi.org/10.1111/febs.15457
N2 - O-methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen-associated molecular pattern. We describe the interaction of O-methylated saccharides with two beta-propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica. The crystal structures of PLL2 and PHL revealed up to 10 out of 14 potential binding sites per protein subunit to be occupied with O-methylated structures. The avidity effect strengthens the interaction by 4 orders of magnitude. PLL2 and PHL also interfere with the early immune response by modulating the production of reactive oxygen species and phenoloxidase activity. Since bacteria from Photorhabdus spp. have a complex life cycle involving pathogenicity towards different hosts, the involvement of PLL2 and PHL might contribute to the pathogen overcoming insect and human immune system defenses in the early stages of infection.
ER -
FUJDIAROVÁ, Eva, Josef HOUSER, Pavel DOBEŠ, Gita PAULÍKOVÁ, Nikolaj KONDAKOV, Leonid KONONOV, Pavel HYRŠL a Michaela WIMMEROVÁ. Heptabladed b-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system. \textit{The FEBS Journal}. Hoboken: Wiley, 2021, roč.~288, č.~4, s.~1343-1365. ISSN~1742-464X. Dostupné z: https://dx.doi.org/10.1111/febs.15457.
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