Evaluation of Microbiome-Host Relationships in the Zebrafish
Gastrointestinal System Reveals Adaptive Immunity Is a Target
of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure

J 2020

Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure

ADAMOVSKÝ, Ondřej; Amanda N. BUERGER; Hana VESPALCOVÁ; Shahadur R. SOHAG; Amy T. HANLON et al.

Základní údaje

Originální název

Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure

Autoři

Vydání

Environmental Science & Technology, Washington, D.C. American Chemical Society, 2020, 0013-936X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.028

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/20:00116075

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

DI(2-ETHYLHEXYL) PHTHALATE; AIRWAY INFLAMMATION; INTESTINAL FAILURE; ENTEROCYTE MASS; TIGHT JUNCTIONS; CELLS; NEUROPEPTIDES; ASSOCIATIONS; CITRULLINE; EXPRESSION

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 4. 2021 18:34, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

To improve physical characteristics of plastics such as flexibility and durability, producers enrich materials with phthalates such as di-2-(ethylhexyl) phthalate (DEHP). DEHP is a high production volume chemical associated with metabolic and immune disruption in animals and humans. To reveal mechanisms implicated in phthalate-related disruption in the gastrointestinal system, male and female zebrafish were fed DEHP (3 ppm) daily for two months. At the transcriptome level, DEHP significantly upregulated gene networks in the intestine associated with helper T cells' (Th1, Th2, and Th17) specific pathways. The activation of gene networks associated with adaptive immunity was linked to the suppression of networks for tight junction, gap junctional intercellular communication, and transmembrane transporters, all of which are precursors for impaired gut integrity and performance. On a class level, DEHP exposure increased Bacteroidia and Gammaproteobacteria and decreased Verrucomicrobiae in both the male and female gastrointestinal system. Further, in males there was a relative increase in Fusobacteriia and Betaproteobacteria and a relative decrease in Saccharibacteria. Predictive algorithms revealed that the functional shift in the microbiome community, and the metabolites they produce, act to modulate intestinal adaptive immunity. This finding suggests that the gut microbiota may contribute to the adverse effects of DEHP on the host by altering metabolites sensed by both intestinal and immune Th cells. Our results suggest that the microbiome-gut-immune axis can be modified by DEHP and emphasize the value of multiomics approaches to study microbiome-host interactions following chemical perturbations.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

707241, interní kód MU

Název: Environmental chemicals as obesogens (Akronym: OBESOGENS)

Investor: Evropská unie, Environmental chemicals as obesogens, MSCA Marie Skłodowska-Curie Actions (Excellent Science)

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90042, velká výzkumná infrastruktura

Název: CESNET II

Přiložené soubory

1672041.pdf

Citovat

ADAMOVSKÝ, Ondřej; Amanda N. BUERGER; Hana VESPALCOVÁ; Shahadur R. SOHAG; Amy T. HANLON; Pamela E. GINN; Serena L. CRAFT; Stanislav SMATANA; Eva BUDINSKÁ; Maria PERSICO; Joseph H., Jr. BISESI a Christopher J. MARTYNIUK. Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure. Environmental Science & Technology. Washington, D.C.: American Chemical Society, 2020, roč. 54, č. 9, s. 5719-5728. ISSN 0013-936X. Dostupné z: https://doi.org/10.1021/acs.est.0c00628.

@article{1672041,
   author = {Adamovský, Ondřej and Buerger, Amanda N. and Vespalcová, Hana and Sohag, Shahadur R. and Hanlon, Amy T. and Ginn, Pamela E. and Craft, Serena L. and Smatana, Stanislav and Budinská, Eva and Persico, Maria and Bisesi, Joseph H., Jr. and Martyniuk, Christopher J.},
   article_location = {Washington, D.C.},
   article_number = {9},
   doi = {https://doi.org/10.1021/acs.est.0c00628},
   keywords = {DI(2-ETHYLHEXYL) PHTHALATE; AIRWAY INFLAMMATION; INTESTINAL FAILURE; ENTEROCYTE MASS; TIGHT JUNCTIONS; CELLS; NEUROPEPTIDES; ASSOCIATIONS; CITRULLINE; EXPRESSION},
   language = {eng},
   issn = {0013-936X},
   journal = {Environmental Science & Technology},
   title = {Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure},
   url = {https://pubs.acs.org/doi/10.1021/acs.est.0c00628},
   volume = {54},
   year = {2020}
}

TY  - JOUR
ID  - 1672041
AU  - Adamovský, Ondřej - Buerger, Amanda N. - Vespalcová, Hana - Sohag, Shahadur R. - Hanlon, Amy T. - Ginn, Pamela E. - Craft, Serena L. - Smatana, Stanislav - Budinská, Eva - Persico, Maria - Bisesi, Joseph H., Jr. - Martyniuk, Christopher J.
PY  - 2020
TI  - Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure
JF  - Environmental Science & Technology
VL  - 54
IS  - 9
SP  - 5719-5728
EP  - 5719-5728
PB  - American Chemical Society
SN  - 0013936X
KW  - DI(2-ETHYLHEXYL) PHTHALATE
KW  - AIRWAY INFLAMMATION
KW  - INTESTINAL FAILURE
KW  - ENTEROCYTE MASS
KW  - TIGHT JUNCTIONS
KW  - CELLS
KW  - NEUROPEPTIDES
KW  - ASSOCIATIONS
KW  - CITRULLINE
KW  - EXPRESSION
UR  - https://pubs.acs.org/doi/10.1021/acs.est.0c00628
L2  - https://pubs.acs.org/doi/10.1021/acs.est.0c00628
N2  - To improve physical characteristics of plastics such as flexibility and durability, producers enrich materials with phthalates such as di-2-(ethylhexyl) phthalate (DEHP). DEHP is a high production volume chemical associated with metabolic and immune disruption in animals and humans. To reveal mechanisms implicated in phthalate-related disruption in the gastrointestinal system, male and female zebrafish were fed DEHP (3 ppm) daily for two months. At the transcriptome level, DEHP significantly upregulated gene networks in the intestine associated with helper T cells' (Th1, Th2, and Th17) specific pathways. The activation of gene networks associated with adaptive immunity was linked to the suppression of networks for tight junction, gap junctional intercellular communication, and transmembrane transporters, all of which are precursors for impaired gut integrity and performance. On a class level, DEHP exposure increased Bacteroidia and Gammaproteobacteria and decreased Verrucomicrobiae in both the male and female gastrointestinal system. Further, in males there was a relative increase in Fusobacteriia and Betaproteobacteria and a relative decrease in Saccharibacteria. Predictive algorithms revealed that the functional shift in the microbiome community, and the metabolites they produce, act to modulate intestinal adaptive immunity. This finding suggests that the gut microbiota may contribute to the adverse effects of DEHP on the host by altering metabolites sensed by both intestinal and immune Th cells. Our results suggest that the microbiome-gut-immune axis can be modified by DEHP and emphasize the value of multiomics approaches to study microbiome-host interactions following chemical perturbations.
ER  -

ADAMOVSKÝ, Ondřej; Amanda N. BUERGER; Hana VESPALCOVÁ; Shahadur R. SOHAG; Amy T. HANLON; Pamela E. GINN; Serena L. CRAFT; Stanislav SMATANA; Eva BUDINSKÁ; Maria PERSICO; Joseph H., Jr. BISESI a Christopher J. MARTYNIUK. Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure. \textit{Environmental Science \&{} Technology}. Washington, D.C.: American Chemical Society, 2020, roč.~54, č.~9, s.~5719-5728. ISSN~0013-936X. Dostupné z: https://doi.org/10.1021/acs.est.0c00628.

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