PLÖSCHNER, Martin, Tomáš TYC a Tomáš ČIŽMÁR. Multimode fibre: a pathway towards deep tissue fluorescence microscopy. Online. In Eggleton, B. J.; Palomba, S. MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS. BELLINGHAM: SPIE-INT SOC OPTICAL ENGINEERING, 2015, s. 1-6. ISBN 978-1-62841-890-3. Dostupné z: https://dx.doi.org/10.1117/12.2202355.
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Základní údaje
Originální název Multimode fibre: a pathway towards deep tissue fluorescence microscopy
Autoři PLÖSCHNER, Martin (203 Česká republika, garant), Tomáš TYC (203 Česká republika, domácí) a Tomáš ČIŽMÁR (203 Česká republika).
Vydání BELLINGHAM, MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS, od s. 1-6, 6 s. 2015.
Nakladatel SPIE-INT SOC OPTICAL ENGINEERING
Další údaje
Originální jazyk angličtina
Typ výsledku Stať ve sborníku
Obor 10306 Optics
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Forma vydání elektronická verze "online"
WWW URL
Kód RIV RIV/00216224:14310/15:00116094
Organizační jednotka Přírodovědecká fakulta
ISBN 978-1-62841-890-3
ISSN 0277-786X
Doi http://dx.doi.org/10.1117/12.2202355
UT WoS 000370723500052
Klíčová slova anglicky multimode optical fibre; digital holography; fluorescence microscopy; micro-endoscopy; wavefront shaping
Štítky rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Marie Šípková, DiS., učo 437722. Změněno: 30. 7. 2020 10:50.
Anotace
Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.
VytisknoutZobrazeno: 7. 9. 2024 23:33