Další formáty:
BibTeX
LaTeX
RIS
@article{1673338, author = {Roudnický, Pavel and Potěšil, David and Zdráhal, Zbyněk and Gelnar, Milan and Kašný, Martin}, article_location = {San Francisco}, article_number = {6}, doi = {http://dx.doi.org/10.1371/journal.pone.0231681}, keywords = {helminths; parasite; peptidase; inhibitor; laser microdissection; mass spectrometry}, language = {eng}, issn = {1932-6203}, journal = {PLOS ONE}, title = {Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)}, url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231681}, volume = {15}, year = {2020} }
TY - JOUR ID - 1673338 AU - Roudnický, Pavel - Potěšil, David - Zdráhal, Zbyněk - Gelnar, Milan - Kašný, Martin PY - 2020 TI - Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea) JF - PLOS ONE VL - 15 IS - 6 SP - 1-15 EP - 1-15 PB - Public Library of Science SN - 19326203 KW - helminths KW - parasite KW - peptidase KW - inhibitor KW - laser microdissection KW - mass spectrometry UR - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231681 L2 - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231681 N2 - Eudiplozoon nipponicum (Goto, 1891) is a hematophagous monogenean ectoparasite which inhabits the gills of the common carp (Cyprinus carpio). Heavy infestation can lead to anemia and in conjunction with secondary bacterial infections cause poor health and eventual death of the host. This study is based on an innovative approach to protein localization which has never been used in parasitology before. Using laser capture microdissection, we dissected particular areas of the parasite body without contaminating the samples by surrounding tissue and in combination with analysis by mass spectrometry obtained tissue-specific proteomes of tegument, intestine, and parenchyma of our model organism, E. nipponicum. We successfully verified the presence of certain functional proteins (e.g. cathepsin L) in tissues where their presence was expected (intestine) and confirmed that there were no traces of these proteins in other tissues (tegument and parenchyma). Additionally, we identified a total of 2,059 proteins, including 72 peptidases and 33 peptidase inhibitors. As expected, the greatest variety was found in the intestine and the lowest variety in the parenchyma. Our results are significant on two levels. Firstly, we demonstrated that one can localize all proteins in one analysis and without using laboratory animals (antibodies for immunolocalization of single proteins). Secondly, this study offers the first complex proteomic data on not only the E. nipponicum but within the whole class of Monogenea, which was from this point of view until recently neglected. ER -
ROUDNICKÝ, Pavel, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR a Martin KAŠNÝ. Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea). \textit{PLOS ONE}. San Francisco: Public Library of Science, 2020, roč.~15, č.~6, s.~1-15. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0231681.
|