Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC
Behavior and Properties

J 2020

Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties

JAUKOVIC, Aleksandra, Desislava ABADJIEVA, Drenka TRIVANOVIC, Elena STOYANOVA, Milena KOSTADINOVA et. al.

Základní údaje

Originální název

Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties

Autoři

JAUKOVIC, Aleksandra (688 Srbsko), Desislava ABADJIEVA (100 Bulharsko), Drenka TRIVANOVIC (688 Srbsko), Elena STOYANOVA (100 Bulharsko), Milena KOSTADINOVA (100 Bulharsko), Shina PASHOVA (100 Bulharsko), Snejana KESTENDJIEVA (100 Bulharsko), Tamara KUKOLJ (688 Srbsko), Michal JEŠETA (203 Česká republika, domácí), Elena KISTANOVA (100 Bulharsko) a Milena MOURDJEVA (100 Bulharsko, garant)

Vydání

Stem Cell Reviews and Reports, NEW YORK, SPRINGER, 2020, 2629-3269

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.739

Kód RIV

RIV/00216224:14110/20:00116197

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s12015-020-10006-9

UT WoS

000549663600001

Klíčová slova anglicky

Mesenchymal stem cells; 3D spheroids microenvironment; Morphological changes; Cell survival; Energy metabolism; Epigenetic regulation; Improved functionality

Štítky

14110411, GPK, GPKgyn, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 10. 2020 13:34, Mgr. Tereza Miškechová

Anotace

V originále

Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.

Citovat

JAUKOVIC, Aleksandra, Desislava ABADJIEVA, Drenka TRIVANOVIC, Elena STOYANOVA, Milena KOSTADINOVA, Shina PASHOVA, Snejana KESTENDJIEVA, Tamara KUKOLJ, Michal JEŠETA, Elena KISTANOVA a Milena MOURDJEVA. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. Stem Cell Reviews and Reports. NEW YORK: SPRINGER, 2020, roč. 16, č. 5, s. 853-875. ISSN 2629-3269. Dostupné z: https://dx.doi.org/10.1007/s12015-020-10006-9.

@article{1674042,
   author = {Jaukovic, Aleksandra and Abadjieva, Desislava and Trivanovic, Drenka and Stoyanova, Elena and Kostadinova, Milena and Pashova, Shina and Kestendjieva, Snejana and Kukolj, Tamara and Ješeta, Michal and Kistanova, Elena and Mourdjeva, Milena},
   article_location = {NEW YORK},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s12015-020-10006-9},
   keywords = {Mesenchymal stem cells; 3D spheroids microenvironment; Morphological changes; Cell survival; Energy metabolism; Epigenetic regulation; Improved functionality},
   language = {eng},
   issn = {2629-3269},
   journal = {Stem Cell Reviews and Reports},
   title = {Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties},
   url = {https://link.springer.com/article/10.1007%2Fs12015-020-10006-9},
   volume = {16},
   year = {2020}
}

TY  - JOUR
ID  - 1674042
AU  - Jaukovic, Aleksandra - Abadjieva, Desislava - Trivanovic, Drenka - Stoyanova, Elena - Kostadinova, Milena - Pashova, Shina - Kestendjieva, Snejana - Kukolj, Tamara - Ješeta, Michal - Kistanova, Elena - Mourdjeva, Milena
PY  - 2020
TI  - Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties
JF  - Stem Cell Reviews and Reports
VL  - 16
IS  - 5
SP  - 853-875
EP  - 853-875
PB  - SPRINGER
SN  - 26293269
KW  - Mesenchymal stem cells
KW  - 3D spheroids microenvironment
KW  - Morphological changes
KW  - Cell survival
KW  - Energy metabolism
KW  - Epigenetic regulation
KW  - Improved functionality
UR  - https://link.springer.com/article/10.1007%2Fs12015-020-10006-9
L2  - https://link.springer.com/article/10.1007%2Fs12015-020-10006-9
N2  - Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.
ER  -

JAUKOVIC, Aleksandra, Desislava ABADJIEVA, Drenka TRIVANOVIC, Elena STOYANOVA, Milena KOSTADINOVA, Shina PASHOVA, Snejana KESTENDJIEVA, Tamara KUKOLJ, Michal JEŠETA, Elena KISTANOVA a Milena MOURDJEVA. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. \textit{Stem Cell Reviews and Reports}. NEW YORK: SPRINGER, 2020, roč.~16, č.~5, s.~853-875. ISSN~2629-3269. Dostupné z: https://dx.doi.org/10.1007/s12015-020-10006-9.

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