Donor-derived DNA variability in fingernails of acute myeloid
leukemia patients after allogeneic hematopoietic stem cell
transplantation detected by direct PCR

J 2020

Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR

ROBEŠOVÁ, Blanka, Marie DRNCOVA, Adam FOLTA, Marta KREJČÍ, František FOLBER et. al.

Základní údaje

Originální název

Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR

Autoři

ROBEŠOVÁ, Blanka (203 Česká republika), Marie DRNCOVA (203 Česká republika), Adam FOLTA (203 Česká republika), Marta KREJČÍ (203 Česká republika, domácí), František FOLBER (203 Česká republika, domácí), Zuzana ŠUSTKOVÁ (203 Česká republika, domácí), Jiří MAYER (203 Česká republika, domácí) a Ivana JEŽÍŠKOVÁ (203 Česká republika, garant, domácí)

Vydání

Bone Marrow Transplantation, London, Nature Publishing Group, 2020, 0268-3369

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30205 Hematology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.483

Kód RIV

RIV/00216224:14110/20:00116201

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1038/s41409-020-0938-x

UT WoS

000531193900001

Klíčová slova anglicky

acute myeloid leukemia; Donor-derived DNA; fingernails; stem cell transplantation

Štítky

14110212, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 9. 2020 12:06, Mgr. Tereza Miškechová

Anotace

V originále

Human fingernails are an important material used to identify individuals in forensic medicine as well as a source of DNA for genetic analyses in different fields of study. Nail clippings have many advantages, such as stability and suitability for long-term storage, easy non-invasive sampling and simple transport. On the other hand, the structure of a fingernail (presence of keratinized cells) requires a more complicated DNA extraction method than DNA extraction from blood cells. Therefore, it is advantageous to use a direct PCR method that does not require a DNA isolation step. This method was used on fingernails for the first time by Tie et al., and by Ottens et al. The benefits of the method are simplicity, reducing the possibility of contamination and time- and cost-saving. We used this innovative method for donor-derived DNA analysis on the fingernails of 30 acute myeloid patients (AML) with long-lasting complete donor chimerism in their peripheral blood after the first allogeneic hematopoietic stem cell transplantation (allo-HSCT).

Citovat

ROBEŠOVÁ, Blanka, Marie DRNCOVA, Adam FOLTA, Marta KREJČÍ, František FOLBER, Zuzana ŠUSTKOVÁ, Jiří MAYER a Ivana JEŽÍŠKOVÁ. Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR. Bone Marrow Transplantation. London: Nature Publishing Group, 2020, roč. 55, č. 6, s. 1021-1022. ISSN 0268-3369. Dostupné z: https://dx.doi.org/10.1038/s41409-020-0938-x.

@article{1674063,
   author = {Robešová, Blanka and Drncova, Marie and Folta, Adam and Krejčí, Marta and Folber, František and Šustková, Zuzana and Mayer, Jiří and Ježíšková, Ivana},
   article_location = {London},
   article_number = {6},
   doi = {http://dx.doi.org/10.1038/s41409-020-0938-x},
   keywords = {acute myeloid leukemia; Donor-derived DNA; fingernails; stem cell transplantation},
   language = {eng},
   issn = {0268-3369},
   journal = {Bone Marrow Transplantation},
   title = {Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR},
   url = {https://www.nature.com/articles/s41409-020-0938-x},
   volume = {55},
   year = {2020}
}

TY  - JOUR
ID  - 1674063
AU  - Robešová, Blanka - Drncova, Marie - Folta, Adam - Krejčí, Marta - Folber, František - Šustková, Zuzana - Mayer, Jiří - Ježíšková, Ivana
PY  - 2020
TI  - Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR
JF  - Bone Marrow Transplantation
VL  - 55
IS  - 6
SP  - 1021-1022
EP  - 1021-1022
PB  - Nature Publishing Group
SN  - 02683369
KW  - acute myeloid leukemia
KW  - Donor-derived DNA
KW  - fingernails
KW  - stem cell transplantation
UR  - https://www.nature.com/articles/s41409-020-0938-x
L2  - https://www.nature.com/articles/s41409-020-0938-x
N2  - Human fingernails are an important material used to identify individuals in forensic medicine as well as a source of DNA for genetic analyses in different fields of study. Nail clippings have many advantages, such as stability and suitability for long-term storage, easy non-invasive sampling and simple transport. On the other hand, the structure of a fingernail (presence of keratinized cells) requires a more complicated DNA extraction method than DNA extraction from blood cells. Therefore, it is advantageous to use a direct PCR method that does not require a DNA isolation step. This method was used on fingernails for the first time by Tie et al., and by Ottens et al. The benefits of the method are simplicity, reducing the possibility of contamination and time- and cost-saving. We used this innovative method for donor-derived DNA analysis on the fingernails of 30 acute myeloid patients (AML) with long-lasting complete donor chimerism in their peripheral blood after the first allogeneic hematopoietic stem cell transplantation (allo-HSCT).
ER  -

ROBEŠOVÁ, Blanka, Marie DRNCOVA, Adam FOLTA, Marta KREJČÍ, František FOLBER, Zuzana ŠUSTKOVÁ, Jiří MAYER a Ivana JEŽÍŠKOVÁ. Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR. \textit{Bone Marrow Transplantation}. London: Nature Publishing Group, 2020, roč.~55, č.~6, s.~1021-1022. ISSN~0268-3369. Dostupné z: https://dx.doi.org/10.1038/s41409-020-0938-x.

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