Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Katarzyna STEFANSKA, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Dorota BUKOWSKA, Maciej ZABEL, Paul MOZDZIAK, Michal NOWICKI, Bartosz KEMPISTY a Pawel ANTOSIK. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. Biomed Research International. London: Hindawi Publishing Corporation, 2020, roč. 2020, FEB 2020, s. 1-15. ISSN 2314-6133. Dostupné z: https://dx.doi.org/10.1155/2020/7120375.

Základní údaje

• Originální název: Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model
• Autoři: WOJTANOWICZ-MARKIEWICZ, Katarzyna (616 Polsko), Magdalena KULUS (616 Polsko), Sandra KNAP (616 Polsko), Ievgenia KOCHEROVA (616 Polsko), Maurycy JANKOWSKI (616 Polsko), Katarzyna STEFANSKA (616 Polsko), Michal JEŠETA (203 Česká republika, domácí), Hanna PIOTROWSKA-KEMPISTY (616 Polsko), Dorota BUKOWSKA (616 Polsko), Maciej ZABEL (616 Polsko), Paul MOZDZIAK (840 Spojené státy), Michal NOWICKI (616 Polsko), Bartosz KEMPISTY (616 Polsko, garant) a Pawel ANTOSIK (616 Polsko).
• Vydání: Biomed Research International, London, Hindawi Publishing Corporation, 2020, 2314-6133.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 10606 Microbiology
• Stát vydavatele: Velká Británie a Severní Irsko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Impakt faktor: Impact factor: 3.411
• Kód RIV: RIV/00216224:14110/20:00116242
• Organizační jednotka: Lékařská fakulta
• Doi: http://dx.doi.org/10.1155/2020/7120375
• UT WoS: 000515519100003
• Klíčová slova anglicky: MESSENGER-RNA; ESTROUS-CYCLE; FEMALE; JUNCTIONS; UTERUS
• Štítky: 14110411, GPK, GPKgyn, rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx's behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.