Expression of Selected Connexin and Aquaporin Genes and
Real-Time Proliferation of Porcine Endometrial Luminal
Epithelial Cells in Primary Culture Model

J 2020

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI et. al.

Základní údaje

Originální název

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

Autoři

WOJTANOWICZ-MARKIEWICZ, Katarzyna (616 Polsko), Magdalena KULUS (616 Polsko), Sandra KNAP (616 Polsko), Ievgenia KOCHEROVA (616 Polsko), Maurycy JANKOWSKI (616 Polsko), Katarzyna STEFANSKA (616 Polsko), Michal JEŠETA (203 Česká republika, domácí), Hanna PIOTROWSKA-KEMPISTY (616 Polsko), Dorota BUKOWSKA (616 Polsko), Maciej ZABEL (616 Polsko), Paul MOZDZIAK (840 Spojené státy), Michal NOWICKI (616 Polsko), Bartosz KEMPISTY (616 Polsko, garant) a Pawel ANTOSIK (616 Polsko)

Vydání

Biomed Research International, London, Hindawi Publishing Corporation, 2020, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.411

Kód RIV

RIV/00216224:14110/20:00116242

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1155/2020/7120375

UT WoS

000515519100003

Klíčová slova anglicky

MESSENGER-RNA; ESTROUS-CYCLE; FEMALE; JUNCTIONS; UTERUS

Štítky

14110411, GPK, GPKgyn, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 8. 2020 13:17, Mgr. Tereza Miškechová

Anotace

V originále

Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx's behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.

Citovat

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Katarzyna STEFANSKA, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Dorota BUKOWSKA, Maciej ZABEL, Paul MOZDZIAK, Michal NOWICKI, Bartosz KEMPISTY a Pawel ANTOSIK. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. Biomed Research International. London: Hindawi Publishing Corporation, 2020, roč. 2020, FEB 2020, s. 1-15. ISSN 2314-6133. Dostupné z: https://dx.doi.org/10.1155/2020/7120375.

@article{1674771,
   author = {WojtanowiczandMarkiewicz, Katarzyna and Kulus, Magdalena and Knap, Sandra and Kocherova, Ievgenia and Jankowski, Maurycy and Stefanska, Katarzyna and Ješeta, Michal and PiotrowskaandKempisty, Hanna and Bukowska, Dorota and Zabel, Maciej and Mozdziak, Paul and Nowicki, Michal and Kempisty, Bartosz and Antosik, Pawel},
   article_location = {London},
   article_number = {FEB 2020},
   doi = {http://dx.doi.org/10.1155/2020/7120375},
   keywords = {MESSENGER-RNA; ESTROUS-CYCLE; FEMALE; JUNCTIONS; UTERUS},
   language = {eng},
   issn = {2314-6133},
   journal = {Biomed Research International},
   title = {Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model},
   url = {http://downloads.hindawi.com/journals/bmri/2020/7120375.pdf},
   volume = {2020},
   year = {2020}
}

TY  - JOUR
ID  - 1674771
AU  - Wojtanowicz-Markiewicz, Katarzyna - Kulus, Magdalena - Knap, Sandra - Kocherova, Ievgenia - Jankowski, Maurycy - Stefanska, Katarzyna - Ješeta, Michal - Piotrowska-Kempisty, Hanna - Bukowska, Dorota - Zabel, Maciej - Mozdziak, Paul - Nowicki, Michal - Kempisty, Bartosz - Antosik, Pawel
PY  - 2020
TI  - Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model
JF  - Biomed Research International
VL  - 2020
IS  - FEB 2020
SP  - 1-15
EP  - 1-15
PB  - Hindawi Publishing Corporation
SN  - 23146133
KW  - MESSENGER-RNA
KW  - ESTROUS-CYCLE
KW  - FEMALE
KW  - JUNCTIONS
KW  - UTERUS
UR  - http://downloads.hindawi.com/journals/bmri/2020/7120375.pdf
L2  - http://downloads.hindawi.com/journals/bmri/2020/7120375.pdf
N2  - Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx's behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.
ER  -

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Katarzyna STEFANSKA, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Dorota BUKOWSKA, Maciej ZABEL, Paul MOZDZIAK, Michal NOWICKI, Bartosz KEMPISTY a Pawel ANTOSIK. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. \textit{Biomed Research International}. London: Hindawi Publishing Corporation, 2020, roč.~2020, FEB 2020, s.~1-15. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2020/7120375.

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