POHANKA, M., Petr ZBOŘIL a J. PIKULA. D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography. Polish journal of chemistry. Warszawa: Państwowe Wydawnictwo Naukowe., 2009, roč. 83, č. 3, s. 415-420. ISSN 0137-5083. |
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@article{1674862, author = {Pohanka, M. and Zbořil, Petr and Pikula, J.}, article_location = {Warszawa}, article_number = {3}, keywords = {baker's yeast; mitochondria; d-lactic acid; isolation}, language = {eng}, issn = {0137-5083}, journal = {Polish journal of chemistry}, title = {D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography}, url = {http://www.ichf.edu.pl/pjch/pj-2009.htm}, volume = {83}, year = {2009} }
TY - JOUR ID - 1674862 AU - Pohanka, M. - Zbořil, Petr - Pikula, J. PY - 2009 TI - D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography JF - Polish journal of chemistry VL - 83 IS - 3 SP - 415-420 EP - 415-420 PB - Państwowe Wydawnictwo Naukowe. SN - 01375083 KW - baker's yeast KW - mitochondria KW - d-lactic acid KW - isolation UR - http://www.ichf.edu.pl/pjch/pj-2009.htm L2 - http://www.ichf.edu.pl/pjch/pj-2009.htm N2 - D-Lactate dehydrogenase (cytochrome) was isolated from baker's yeasts (Saccharomyces cerevisiae) using fast protein liquid chromatography. Mitochondria from disrupted cells were separated and enzymes released by Triton X-100. Purification was performed by fast protein liquid chromatography on hydroxylapatite and in another separate experiment on AH-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid (SHCA). Although purification on hydtoxylapatite provided approachable samples with activities for only D-lactic acid, chromatography on SHCA seemed to be better because a purified fraction of enzyme with the specific activity of 3.38 nkat/mg was obtained. Another problem considered was the usefulness of the combination of chromatography on hydroxylapatite and SHCA providing a fraction of D-lactic acid with specific activity of 5.71 nkat/mg and no cross reactivity for L-lactic acid. ER -
POHANKA, M., Petr ZBOŘIL a J. PIKULA. D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography. \textit{Polish journal of chemistry}. Warszawa: Pa\'nstwowe Wydawnictwo Naukowe., 2009, roč.~83, č.~3, s.~415-420. ISSN~0137-5083.
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