Monitoring of embryonic stem cell differentiation trajectories
by intact cell mass spectrometry

k 2020

Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry

VAŇHARA, Petr, Andreas SCHNAPP, Lukáš MORÁŇ, Lukáš PEČINKA, Hana KOTASOVÁ et. al.

Základní údaje

Originální název

Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry

Autoři

VAŇHARA, Petr, Andreas SCHNAPP, Lukáš MORÁŇ, Lukáš PEČINKA, Hana KOTASOVÁ, Vendula PELKOVÁ, Josef HAVEL a Aleš HAMPL

Vydání

68th Annual Conference of American Society for Mass Spectrometry, Houston, TX, USA, 2020

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Klíčová slova česky

embryonální kmenové buňky; hmotnostní spektrometrie; monitoring; diferenciace

Klíčová slova anglicky

embryonic stem cells; mass spectrometry; monitoring; differentiation

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 10. 2020 14:13, Mgr. Lukáš Pečinka, Ph.D.

Anotace

V originále

Human embryonic stem cells (hESCs) represent a promising tool for clinical applications. hESCs differentiation to relevant cell types is a gradual process, where final phenotype is achieved. However, substantial heterogeneity in differentiation process may produce aberrant cells with unwanted properties, such as lack of functional phenotype, or propensity to cancer growth. Early lung progenitors (ELEPs) are direct precursors of mature lung cells (pneumocytes) that differentiate from hESCs under specific conditions through several stages. Differentiation pathway can be outlined specifically by molecular markers, but an unbiased, sensitive and robust tool for discrimination of ELEPs from pluripotent or transitional stages is still missing. Here we demonstrate that intact cell MALDI TOF mass spectrometry may provide such a tool for quality control.

Citovat

VAŇHARA, Petr, Andreas SCHNAPP, Lukáš MORÁŇ, Lukáš PEČINKA, Hana KOTASOVÁ, Vendula PELKOVÁ, Josef HAVEL a Aleš HAMPL. Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry. In 68th Annual Conference of American Society for Mass Spectrometry, Houston, TX, USA. 2020.

@proceedings{1675660,
   author = {Vaňhara, Petr and Schnapp, Andreas and Moráň, Lukáš and Pečinka, Lukáš and Kotasová, Hana and Pelková, Vendula and Havel, Josef and Hampl, Aleš},
   booktitle = {68th Annual Conference of American Society for Mass Spectrometry, Houston, TX, USA},
   keywords = {embryonic stem cells; mass spectrometry; monitoring; differentiation},
   language = {eng},
   title = {Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry},
   year = {2020}
}

TY  - CONF
ID  - 1675660
AU  - Vaňhara, Petr - Schnapp, Andreas - Moráň, Lukáš - Pečinka, Lukáš - Kotasová, Hana - Pelková, Vendula - Havel, Josef - Hampl, Aleš
PY  - 2020
TI  - Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry
KW  - embryonic stem cells
KW  - mass spectrometry
KW  - monitoring
KW  - differentiation
N2  - Human embryonic stem cells (hESCs) represent a promising tool for clinical applications. hESCs differentiation to relevant cell types is a gradual process, where final phenotype is achieved. However, substantial heterogeneity in differentiation process may produce aberrant cells with unwanted properties, such as lack of functional phenotype, or propensity to cancer growth. Early lung progenitors (ELEPs) are direct precursors of mature lung cells (pneumocytes) that differentiate from hESCs under specific conditions through several stages. Differentiation pathway can be outlined specifically by molecular markers, but an unbiased, sensitive and robust tool for discrimination of ELEPs from pluripotent or transitional stages is still missing. Here we demonstrate that intact cell MALDI TOF mass spectrometry may provide such a tool for quality control.
ER  -

VAŇHARA, Petr, Andreas SCHNAPP, Lukáš MORÁŇ, Lukáš PEČINKA, Hana KOTASOVÁ, Vendula PELKOVÁ, Josef HAVEL a Aleš HAMPL. Monitoring of embryonic stem cell differentiation trajectories by intact cell mass spectrometry. In \textit{68th Annual Conference of American Society for Mass Spectrometry, Houston, TX, USA}. 2020.

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