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@inbook{1675802, author = {Schořová, Šárka and Fajkus, Jiří and Procházková Schrumpfová, Petra}, address = {New York, USA}, booktitle = {The Nucleus - Methods in Molecular Biology}, doi = {http://dx.doi.org/10.1007/978-1-0716-0763-3_11}, editor = {Ronald Hancock}, keywords = {Methods; protein-protein interaction; DNA-protein interaction; Bimolecular fluorescence complementation; Co-immunoprecipitation; Yeast two-hybrid system; ChIP for low-abundance proteins}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York, USA}, isbn = {978-1-0716-0762-6}, pages = {139-167}, publisher = {Springer Nature, Humana Press}, title = {Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres}, url = {https://doi.org/10.1007/978-1-0716-0763-3_11}, year = {2020} }
TY - CHAP ID - 1675802 AU - Schořová, Šárka - Fajkus, Jiří - Procházková Schrumpfová, Petra PY - 2020 TI - Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres VL - Third Edition PB - Springer Nature, Humana Press CY - New York, USA SN - 9781071607626 KW - Methods KW - protein-protein interaction KW - DNA-protein interaction KW - Bimolecular fluorescence complementation KW - Co-immunoprecipitation KW - Yeast two-hybrid system KW - ChIP for low-abundance proteins UR - https://doi.org/10.1007/978-1-0716-0763-3_11 N2 - Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization) ER -
SCHOŘOVÁ, Šárka, Jiří FAJKUS a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres. In Ronald Hancock. \textit{The Nucleus - Methods in Molecular Biology}. New York, USA: Springer Nature, Humana Press, 2020, s.~139-167. Third Edition. ISBN~978-1-0716-0762-6. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-0763-3\_{}11.
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