Optimized Detection of Protein-Protein and Protein-DNA
Interactions, with Particular Application to Plant Telomeres

C 2020

Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

SCHOŘOVÁ, Šárka, Jiří FAJKUS a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ

Základní údaje

Originální název

Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres

Autoři

SCHOŘOVÁ, Šárka (203 Česká republika, domácí), Jiří FAJKUS (203 Česká republika, garant, domácí) a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ (203 Česká republika, domácí)

Vydání

New York, USA, The Nucleus - Methods in Molecular Biology, od s. 139-167, 29 s. Third Edition, 2020

Nakladatel

Springer Nature, Humana Press

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14310/20:00114288

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-0716-0762-6

DOI

http://dx.doi.org/10.1007/978-1-0716-0763-3_11

UT WoS

000681366300012

Klíčová slova anglicky

Methods; protein-protein interaction; DNA-protein interaction; Bimolecular fluorescence complementation; Co-immunoprecipitation; Yeast two-hybrid system; ChIP for low-abundance proteins

Štítky

rivok, topvydavatel

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 9. 2021 14:33, Mgr. Marie Šípková, DiS.

Anotace

V originále

Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization)

Návaznosti

GA17-09644S, projekt VaV

Název: Molekulární podstata evolučních přeměn telomer u rostlin řádu Asparagales

Investor: Grantová agentura ČR, Molekulární podstata evolučních přeměn telomer u rostlin řádu Asparagales

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SCHOŘOVÁ, Šárka, Jiří FAJKUS a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres. In Ronald Hancock. The Nucleus - Methods in Molecular Biology. New York, USA: Springer Nature, Humana Press, 2020, s. 139-167. Third Edition. ISBN 978-1-0716-0762-6. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-0763-3_11.

@inbook{1675802,
   author = {Schořová, Šárka and Fajkus, Jiří and Procházková Schrumpfová, Petra},
   address = {New York, USA},
   booktitle = {The Nucleus - Methods in Molecular Biology},
   doi = {http://dx.doi.org/10.1007/978-1-0716-0763-3_11},
   editor = {Ronald Hancock},
   keywords = {Methods; protein-protein interaction; DNA-protein interaction; Bimolecular fluorescence complementation; Co-immunoprecipitation; Yeast two-hybrid system; ChIP for low-abundance proteins},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York, USA},
   isbn = {978-1-0716-0762-6},
   pages = {139-167},
   publisher = {Springer Nature, Humana Press},
   title = {Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres},
   url = {https://doi.org/10.1007/978-1-0716-0763-3_11},
   year = {2020}
}

TY  - CHAP
ID  - 1675802
AU  - Schořová, Šárka - Fajkus, Jiří - Procházková Schrumpfová, Petra
PY  - 2020
TI  - Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres
VL  - Third Edition
PB  - Springer Nature, Humana Press
CY  - New York, USA
SN  - 9781071607626
KW  - Methods
KW  - protein-protein interaction
KW  - DNA-protein interaction
KW  - Bimolecular fluorescence complementation
KW  - Co-immunoprecipitation
KW  - Yeast two-hybrid system
KW  - ChIP for low-abundance proteins
UR  - https://doi.org/10.1007/978-1-0716-0763-3_11
N2  - Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. Here we describe optimized methods and their mutual combinations for this purpose: bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP), yeast two-hybrid systems (Y2H), and chromatin immunoprecipitation (ChIP). These improved protocols detect trimeric complexes in which two proteins of interest interact indirectly via a protein sandwiched between them. They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. Here we describe these methods and their application to map interactions of several telomere- and telomerase-associated proteins and to purify a sufficient amount of chromatin from Arabidopsis thaliana for further investigations (e.g., next-generation sequencing, hybridization)
ER  -

SCHOŘOVÁ, Šárka, Jiří FAJKUS a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. Optimized Detection of Protein-Protein and Protein-DNA Interactions, with Particular Application to Plant Telomeres. In Ronald Hancock. \textit{The Nucleus - Methods in Molecular Biology}. New York, USA: Springer Nature, Humana Press, 2020, s.~139-167. Third Edition. ISBN~978-1-0716-0762-6. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-0763-3\_{}11.

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