Cell-surface receptors enable perception of extracellular
cytokinins

J 2020

Cell-surface receptors enable perception of extracellular cytokinins

ANTONIADI, Ioanna, Ondřej NOVÁK, Zuzana GELOVÁ, Alexander JOHNSON, Ondřej PLÍHAL et. al.

Základní údaje

Originální název

Cell-surface receptors enable perception of extracellular cytokinins

Autoři

ANTONIADI, Ioanna, Ondřej NOVÁK, Zuzana GELOVÁ (203 Česká republika, domácí), Alexander JOHNSON, Ondřej PLÍHAL, Radim SIMERSKÝ, Václav MIK, Thomas VAIN, Eduardo MATEO-BONMATÍ, Michal KARADY, Markéta PERNISOVÁ (203 Česká republika, garant, domácí), Lenka PLAČKOVÁ, Korawit OPASSATHIAN, Jan HEJÁTKO (203 Česká republika, domácí), Stéphanie ROBERT, Jiří FRIML, Karel DOLEŽAL, Karin LJUNG a Colin TURNBULL

Vydání

NATURE COMMUNICATIONS, Nature Publishing Group, 2020, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 14.919

Kód RIV

RIV/00216224:14740/20:00114289

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41467-020-17700-9

UT WoS

000567931000001

Klíčová slova anglicky

cytokinin signalling

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 8. 2023 09:28, Mgr. Markéta Pernisová, Ph.D.

Anotace

V originále

Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.

Návaznosti

EMBO Short-Term Fellowship Number 7034, interní kód MU

Název: Deciphering the mechanism by which different cytokinin types stimulate cytokinin signalling

Investor: EMBO (European Molecular Biology Organization), Deciphering the mechanism by which different cytokinin types stimulate cytokinin signalling

GP14-30004P, projekt VaV

Název: Cytokininy řízené molekulární mechanizmy regulující identitu orgánů u Arabidopsis.

Investor: Grantová agentura ČR, Cytokininy řízené molekulární mechanizmy regulující identitu orgánů u Arabidopsis.

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ANTONIADI, Ioanna, Ondřej NOVÁK, Zuzana GELOVÁ, Alexander JOHNSON, Ondřej PLÍHAL, Radim SIMERSKÝ, Václav MIK, Thomas VAIN, Eduardo MATEO-BONMATÍ, Michal KARADY, Markéta PERNISOVÁ, Lenka PLAČKOVÁ, Korawit OPASSATHIAN, Jan HEJÁTKO, Stéphanie ROBERT, Jiří FRIML, Karel DOLEŽAL, Karin LJUNG a Colin TURNBULL. Cell-surface receptors enable perception of extracellular cytokinins. NATURE COMMUNICATIONS. Nature Publishing Group, 2020, roč. 11, č. 4284, s. 1-10. ISSN 2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-020-17700-9.

@article{1675918,
   author = {Antoniadi, Ioanna and Novák, Ondřej and Gelová, Zuzana and Johnson, Alexander and Plíhal, Ondřej and Simerský, Radim and Mik, Václav and Vain, Thomas and MateoandBonmatí, Eduardo and Karady, Michal and Pernisová, Markéta and Plačková, Lenka and Opassathian, Korawit and Hejátko, Jan and Robert, Stéphanie and Friml, Jiří and Doležal, Karel and Ljung, Karin and Turnbull, Colin},
   article_number = {4284},
   doi = {http://dx.doi.org/10.1038/s41467-020-17700-9},
   keywords = {cytokinin signalling},
   language = {eng},
   issn = {2041-1723},
   journal = {NATURE COMMUNICATIONS},
   title = {Cell-surface receptors enable perception of extracellular cytokinins},
   url = {https://www.nature.com/articles/s41467-020-17700-9},
   volume = {11},
   year = {2020}
}

TY  - JOUR
ID  - 1675918
AU  - Antoniadi, Ioanna - Novák, Ondřej - Gelová, Zuzana - Johnson, Alexander - Plíhal, Ondřej - Simerský, Radim - Mik, Václav - Vain, Thomas - Mateo-Bonmatí, Eduardo - Karady, Michal - Pernisová, Markéta - Plačková, Lenka - Opassathian, Korawit - Hejátko, Jan - Robert, Stéphanie - Friml, Jiří - Doležal, Karel - Ljung, Karin - Turnbull, Colin
PY  - 2020
TI  - Cell-surface receptors enable perception of extracellular cytokinins
JF  - NATURE COMMUNICATIONS
VL  - 11
IS  - 4284
SP  - 1-10
EP  - 1-10
PB  - Nature Publishing Group
SN  - 20411723
KW  - cytokinin signalling
UR  - https://www.nature.com/articles/s41467-020-17700-9
L2  - https://www.nature.com/articles/s41467-020-17700-9
N2  - Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.
ER  -

ANTONIADI, Ioanna, Ondřej NOVÁK, Zuzana GELOVÁ, Alexander JOHNSON, Ondřej PLÍHAL, Radim SIMERSKÝ, Václav MIK, Thomas VAIN, Eduardo MATEO-BONMATÍ, Michal KARADY, Markéta PERNISOVÁ, Lenka PLAČKOVÁ, Korawit OPASSATHIAN, Jan HEJÁTKO, Stéphanie ROBERT, Jiří FRIML, Karel DOLEŽAL, Karin LJUNG a Colin TURNBULL. Cell-surface receptors enable perception of extracellular cytokinins. \textit{NATURE COMMUNICATIONS}. Nature Publishing Group, 2020, roč.~11, č.~4284, s.~1-10. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-020-17700-9.

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