SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For

D 2011

SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress

NAGY, Livia, Tuende ANGYAL, Geza NAGY, Matsumoto AKIKO, Jan PŘIBYL et. al.

Basic information

Original name

SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress

Authors

NAGY, Livia, Tuende ANGYAL, Geza NAGY, Matsumoto AKIKO, Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, belonging to the institution)

Edition

SETUBAL, BIODEVICES 2011, p. 359-362, 4 pp. 2011

Publisher

SCITEPRESS

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

20602 Medical laboratory technology ;

Country of publisher

Portugal

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

Organization unit

Faculty of Science

ISBN

978-989-8425-37-9

UT WoS

000308513400068

Keywords in English

Alcohol induced oxidative stress; Reactive oxygen species (ROS); and Electrochemical detection

Abstract

V originále

The role of reactive oxidizing species (ROS) is proved within numerous physiological processes, including aging, signal transduction and some kind of immune functions. Nowadays ROS and oxidative stress gain increasing attention in connection with a wide spectrum of diseases. In case of Asian people, the enzyme taking part in the ethanol metabolism, the aldehyde dehydrogenase is absent or mutated, that can result in liver tissue damage upon extensive alcohol consumption. Most of the ROS species are electrochemically active therefore the applications of electrochemical methods are the most promising for in situ or in vivo monitoring or quantification of them. In our work development and improvements of selective and sensitive method for electrochemical detection of these molecules and radicals are attempted. We prepared ultra thin size-exclusion layer by electropolymerization of m-phenylenediamine monomer on the surface of the Pt working electrode to ensure its selectivity. We have worked out the optimal circumstances for the selective layer preparation and tested its stability and function. In order to enhance the sensitivity of ROS detection a new amperometric method, the periodically interrupted amperometry (PIA) was developed and applied. With this approach we succeeded selective and sensitive detection of H2O2 in vitro.

Citovat

NAGY, Livia, Tuende ANGYAL, Geza NAGY, Matsumoto AKIKO, Jan PŘIBYL and Petr SKLÁDAL. SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress. Online. In Vieira, P.; Fred, A.; Filipe, J.; Gamboa, H. BIODEVICES 2011. SETUBAL: SCITEPRESS, 2011, p. 359-362. ISBN 978-989-8425-37-9.

@inproceedings{1676297,
   author = {Nagy, Livia and Angyal, Tuende and Nagy, Geza and Akiko, Matsumoto and Přibyl, Jan and Skládal, Petr},
   address = {SETUBAL},
   booktitle = {BIODEVICES 2011},
   editor = {Vieira, P.; Fred, A.; Filipe, J.; Gamboa, H.},
   keywords = {Alcohol induced oxidative stress; Reactive oxygen species (ROS); and Electrochemical detection},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {SETUBAL},
   isbn = {978-989-8425-37-9},
   pages = {359-362},
   publisher = {SCITEPRESS},
   title = {SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress},
   url = {https://www.scitepress.org/Papers/2011/31573/31573.pdf},
   year = {2011}
}

TY  - JOUR
ID  - 1676297
AU  - Nagy, Livia - Angyal, Tuende - Nagy, Geza - Akiko, Matsumoto - Přibyl, Jan - Skládal, Petr
PY  - 2011
TI  - SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For "in vivo" Measurement of ROS in Ethanol Induced Stress
PB  - SCITEPRESS
CY  - SETUBAL
SN  - 9789898425379
KW  - Alcohol induced oxidative stress
KW  - Reactive oxygen species (ROS)
KW  - and Electrochemical detection
UR  - https://www.scitepress.org/Papers/2011/31573/31573.pdf
L2  - https://www.scitepress.org/Papers/2011/31573/31573.pdf
N2  - The role of reactive oxidizing species (ROS) is proved within numerous physiological processes, including aging, signal transduction and some kind of immune functions. Nowadays ROS and oxidative stress gain increasing attention in connection with a wide spectrum of diseases. In case of Asian people, the enzyme taking part in the ethanol metabolism, the aldehyde dehydrogenase is absent or mutated, that can result in liver tissue damage upon extensive alcohol consumption. Most of the ROS species are electrochemically active therefore the applications of electrochemical methods are the most promising for in situ or in vivo monitoring or quantification of them. In our work development and improvements of selective and sensitive method for electrochemical detection of these molecules and radicals are attempted. We prepared ultra thin size-exclusion layer by electropolymerization of m-phenylenediamine monomer on the surface of the Pt working electrode to ensure its selectivity. We have worked out the optimal circumstances for the selective layer preparation and tested its stability and function. In order to enhance the sensitivity of ROS detection a new amperometric method, the periodically interrupted amperometry (PIA) was developed and applied. With this approach we succeeded selective and sensitive detection of H2O2 in vitro.
ER  -

NAGY, Livia, Tuende ANGYAL, Geza NAGY, Matsumoto AKIKO, Jan PŘIBYL and Petr SKLÁDAL. SENSITIVE ELECTROCHEMICAL METHOD DEVELOPMENT For ''in vivo'' Measurement of ROS in Ethanol Induced Stress. Online. In Vieira, P.; Fred, A.; Filipe, J.; Gamboa, H. \textit{BIODEVICES 2011}. SETUBAL: SCITEPRESS, 2011, p.~359-362. ISBN~978-989-8425-37-9.

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