| ĎURČ, Pavol, František FORET, Lukáš HOMOLA, Miriam MALÁ, Eva POKOJOVÁ, Hana VINOHRADSKÁ, Milan DASTYCH, Dagmar KRAUSOVÁ, D. NAGY, O. BEDE, P. DREVINEK, V. SKALICKA and Petr KUBÁŇ. Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis. Pediatric Pulmonology. 111 RIVER ST, HOBOKEN 07030-5774,: WILEY, 2020, vol. 55, No 7, p. 1653-1660. ISSN 8755-6863. Available from: https://dx.doi.org/10.1002/ppul.24758. |
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@article{1677134,
author = {Ďurč, Pavol and Foret, František and Homola, Lukáš and Malá, Miriam and Pokojová, Eva and Vinohradská, Hana and Dastych, Milan and Krausová, Dagmar and Nagy, D. and Bede, O. and Drevinek, P. and Skalicka, V. and Kubáň, Petr},
article_location = {111 RIVER ST, HOBOKEN 07030-5774,},
article_number = {7},
doi = {http://dx.doi.org/10.1002/ppul.24758},
keywords = {capillary electrophoresis; cystic fibrosis; diagnosis; ion ratio; screening; skin wipe test; sweat test},
language = {eng},
issn = {8755-6863},
journal = {Pediatric Pulmonology},
title = {Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/ppul.24758},
volume = {55},
year = {2020}
}
TY - JOUR
ID - 1677134
AU - Ďurč, Pavol - Foret, František - Homola, Lukáš - Malá, Miriam - Pokojová, Eva - Vinohradská, Hana - Dastych, Milan - Krausová, Dagmar - Nagy, D. - Bede, O. - Drevinek, P. - Skalicka, V. - Kubáň, Petr
PY - 2020
TI - Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis
JF - Pediatric Pulmonology
VL - 55
IS - 7
SP - 1653-1660
EP - 1653-1660
PB - WILEY
SN - 87556863
KW - capillary electrophoresis
KW - cystic fibrosis
KW - diagnosis
KW - ion ratio
KW - screening
KW - skin wipe test
KW - sweat test
UR - https://onlinelibrary.wiley.com/doi/full/10.1002/ppul.24758
L2 - https://onlinelibrary.wiley.com/doi/full/10.1002/ppul.24758
N2 - Objective To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). Study Design Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 mu L of deionized (DI) water and extracted into 400 mu L of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl-/K(+)and (Cl- + Na+)/K(+)ion ratios were evaluated. Chloride concentrations from Macroduct ST were analyzed coulometrically. Results Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl-/K(+)and (Cl- + Na+)/K+, from the SWT samples and Cl(-)values from the ST samples were evaluated to diagnose CF. Sensitivity of the SWT method using the Cl-/K(+)ratio (cutoff value 3.9) was 93.9%, compared to 99.1% when using the (Cl- + Na+)/K(+)ratio (cutoff value 5.0) and 98.3% in using Macroduct Cl-(cutoff value higher or equal to 60 mmol/L). The methods' specificities were 97.8%, 94.0%, and 100.0%, respectively. Conclusions The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.
ER -
ĎURČ, Pavol, František FORET, Lukáš HOMOLA, Miriam MALÁ, Eva POKOJOVÁ, Hana VINOHRADSKÁ, Milan DASTYCH, Dagmar KRAUSOVÁ, D. NAGY, O. BEDE, P. DREVINEK, V. SKALICKA and Petr KUBÁŇ. Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis. \textit{Pediatric Pulmonology}. 111 RIVER ST, HOBOKEN 07030-5774,: WILEY, 2020, vol.~55, No~7, p.~1653-1660. ISSN~8755-6863. Available from: https://dx.doi.org/10.1002/ppul.24758.
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