J 2020

High-throughput sequencing of T-cell receptor alpha chain clonal rearrangements at the DNA level in lymphoid malignancies

KOMKOV, Alexander, Anna MIROSHNICHENKOVA, Gaiaz NUGMANOV, Alexander POPOV, Mikhail POGORELYY et. al.

Základní údaje

Originální název

High-throughput sequencing of T-cell receptor alpha chain clonal rearrangements at the DNA level in lymphoid malignancies

Autoři

KOMKOV, Alexander (643 Rusko, garant), Anna MIROSHNICHENKOVA (643 Rusko), Gaiaz NUGMANOV (643 Rusko), Alexander POPOV (643 Rusko), Mikhail POGORELYY (643 Rusko), Eva ZAPLETALOVÁ (203 Česká republika, domácí), Hana JELINKOVA (203 Česká republika), Šárka POSPÍŠILOVÁ (203 Česká republika, domácí), Yuri LEBEDEV (643 Rusko), Dmitriy CHUDAKOV (642 Rumunsko, domácí), Yulia OLSHANSKAYA (643 Rusko), Karla PLEVOVÁ (203 Česká republika, domácí), Michael MASCHAN (643 Rusko) a Ilgar MAMEDOV (643 Rusko, domácí)

Vydání

British Journal of Haematology, England, Wiley-Blackwell, 2020, 0007-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30205 Hematology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 6.998

Kód RIV

RIV/00216224:14740/20:00116416

Organizační jednotka

Středoevropský technologický institut

UT WoS

000516520300019

Klíčová slova anglicky

T-cell receptor alpha chain; clonal rearrangements; acute lymphoblastic leukaemia; lymphoproliferative disorders; high-throughput sequencing

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 3. 3. 2021 14:36, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Rearrangements of T- and B-cell receptor (TCR and BCR) genes are useful markers for clonality assessment as well as for minimal residual disease (MRD) monitoring during the treatment of haematological malignancies. Currently, rearrangements of three out of four TCR and all BCR loci are used for this purpose. The fourth TCR gene, TRA, has not been used so far due to the lack of a method for its rearrangement detection in genomic DNA. Here we propose the first high-throughput sequencing based method for the identification of clonal TRA gene rearrangements at the DNA level. The method is based on target amplification of the rearranged TRA locus using an advanced multiplex polymerase chain reaction system and high-throughput sequencing, and has been tested on DNA samples from peripheral blood of healthy donors. Combinations of all functional V- and J-segments were detected, indicating the high sensitivity of the method. Additionally, we identified clonal TRA rearrangements in 57 out of 112 tested DNA samples of patients with various T-lineage lymphoproliferative disorders. The method fills the existing gap in utilizing the TRA gene for a wide range of studies, including clonality assessment, MRD monitoring and clonal evolution analysis in different lymphoid malignancies.