A retrospective study on nested PCR detection of syphilis
treponemes in clinical samples: PCR detection contributes to
the diagnosis of syphilis in patients with seronegative and
serodiscrepant results

J 2020

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL et. al.

Základní údaje

Originální název

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

Autoři

VRBOVÁ, Eliška (203 Česká republika, domácí), Lenka MIKALOVÁ (203 Česká republika, domácí), Linda GRILLOVÁ (203 Česká republika, domácí), Petra POSPÍŠILOVÁ (203 Česká republika, domácí), Radim STRNADEL (203 Česká republika), Eliška DASTYCHOVÁ (203 Česká republika, domácí), Martina KOJANOVA (203 Česká republika), Miluse KREIDLOVA (203 Česká republika), Daniela VANOUSOVA (203 Česká republika), Filip ROB (203 Česká republika), Premysl PROCHAZKA (203 Česká republika), Alena KRCHŇÁKOVÁ (203 Česká republika), Vladimír VAŠKŮ (203 Česká republika, domácí), Vladana WOZNICOVÁ (203 Česká republika, domácí), Monika DVOŘÁKOVÁ HEROLDOVÁ (203 Česká republika, domácí), Ivana KUKLOVA (203 Česká republika), Hana ZAKOUCKA (203 Česká republika) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

Plos one, San Francisco, Public Library of Science, 2020, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.240

Kód RIV

RIV/00216224:14110/20:00116428

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0237949

UT WoS

000564315600003

Klíčová slova anglicky

syphilis treponeme; PCR detection

Štítky

14110113, 14110125, 14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2021 12:32, Mgr. Tereza Miškechová

Anotace

V originále

Syphilis, caused byTreponema pallidumssp.pallidum(TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.

Návaznosti

MUNI/A/0951/2019, interní kód MU

Název: Buněčná a molekulární biologie pro Biomedicínské vědy

Investor: Masarykova univerzita, Buněčná a molekulární biologie pro Biomedicínské vědy, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NV17-31333A, projekt VaV

Název: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Martina KOJANOVA, Miluse KREIDLOVA, Daniela VANOUSOVA, Filip ROB, Premysl PROCHAZKA, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ, Vladana WOZNICOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA a David ŠMAJS. A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results. Plos one. San Francisco: Public Library of Science, 2020, roč. 15, č. 8, s. 1-11. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0237949.

@article{1679128,
   author = {Vrbová, Eliška and Mikalová, Lenka and Grillová, Linda and Pospíšilová, Petra and Strnadel, Radim and Dastychová, Eliška and Kojanova, Martina and Kreidlova, Miluse and Vanousova, Daniela and Rob, Filip and Prochazka, Premysl and Krchňáková, Alena and Vašků, Vladimír and Woznicová, Vladana and Dvořáková Heroldová, Monika and Kuklova, Ivana and Zakoucka, Hana and Šmajs, David},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0237949},
   keywords = {syphilis treponeme; PCR detection},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results},
   url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable},
   volume = {15},
   year = {2020}
}

TY  - JOUR
ID  - 1679128
AU  - Vrbová, Eliška - Mikalová, Lenka - Grillová, Linda - Pospíšilová, Petra - Strnadel, Radim - Dastychová, Eliška - Kojanova, Martina - Kreidlova, Miluse - Vanousova, Daniela - Rob, Filip - Prochazka, Premysl - Krchňáková, Alena - Vašků, Vladimír - Woznicová, Vladana - Dvořáková Heroldová, Monika - Kuklova, Ivana - Zakoucka, Hana - Šmajs, David
PY  - 2020
TI  - A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results
JF  - Plos one
VL  - 15
IS  - 8
SP  - 1-11
EP  - 1-11
PB  - Public Library of Science
SN  - 19326203
KW  - syphilis treponeme
KW  - PCR detection
UR  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable
L2  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable
N2  - Syphilis, caused byTreponema pallidumssp.pallidum(TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.
ER  -

VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Martina KOJANOVA, Miluse KREIDLOVA, Daniela VANOUSOVA, Filip ROB, Premysl PROCHAZKA, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ, Vladana WOZNICOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA a David ŠMAJS. A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results. \textit{Plos one}. San Francisco: Public Library of Science, 2020, roč.~15, č.~8, s.~1-11. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0237949.

Podrobný výpis o publikaci