A retrospective study on nested PCR detection of syphilis
treponemes in clinical samples: PCR detection contributes to
the diagnosis of syphilis in patients with seronegative and
serodiscrepant results

J 2020

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL et. al.

Basic information

Original name

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

Authors

VRBOVÁ, Eliška (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), Linda GRILLOVÁ (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Radim STRNADEL (203 Czech Republic), Eliška DASTYCHOVÁ (203 Czech Republic, belonging to the institution), Martina KOJANOVA (203 Czech Republic), Miluse KREIDLOVA (203 Czech Republic), Daniela VANOUSOVA (203 Czech Republic), Filip ROB (203 Czech Republic), Premysl PROCHAZKA (203 Czech Republic), Alena KRCHŇÁKOVÁ (203 Czech Republic), Vladimír VAŠKŮ (203 Czech Republic, belonging to the institution), Vladana WOZNICOVÁ (203 Czech Republic, belonging to the institution), Monika DVOŘÁKOVÁ HEROLDOVÁ (203 Czech Republic, belonging to the institution), Ivana KUKLOVA (203 Czech Republic), Hana ZAKOUCKA (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos one, San Francisco, Public Library of Science, 2020, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.240

RIV identification code

RIV/00216224:14110/20:00116428

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0237949

UT WoS

000564315600003

Keywords in English

syphilis treponeme; PCR detection

Abstract

V originále

Syphilis, caused byTreponema pallidumssp.pallidum(TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.

Links

MUNI/A/0951/2019, interní kód MU

Name: Buněčná a molekulární biologie pro Biomedicínské vědy

Investor: Masaryk University, Category A

NV17-31333A, research and development project

Name: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Martina KOJANOVA, Miluse KREIDLOVA, Daniela VANOUSOVA, Filip ROB, Premysl PROCHAZKA, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ, Vladana WOZNICOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA and David ŠMAJS. A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results. Plos one. San Francisco: Public Library of Science, 2020, vol. 15, No 8, p. 1-11. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0237949.

@article{1679128,
   author = {Vrbová, Eliška and Mikalová, Lenka and Grillová, Linda and Pospíšilová, Petra and Strnadel, Radim and Dastychová, Eliška and Kojanova, Martina and Kreidlova, Miluse and Vanousova, Daniela and Rob, Filip and Prochazka, Premysl and Krchňáková, Alena and Vašků, Vladimír and Woznicová, Vladana and Dvořáková Heroldová, Monika and Kuklova, Ivana and Zakoucka, Hana and Šmajs, David},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0237949},
   keywords = {syphilis treponeme; PCR detection},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results},
   url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable},
   volume = {15},
   year = {2020}
}

TY  - JOUR
ID  - 1679128
AU  - Vrbová, Eliška - Mikalová, Lenka - Grillová, Linda - Pospíšilová, Petra - Strnadel, Radim - Dastychová, Eliška - Kojanova, Martina - Kreidlova, Miluse - Vanousova, Daniela - Rob, Filip - Prochazka, Premysl - Krchňáková, Alena - Vašků, Vladimír - Woznicová, Vladana - Dvořáková Heroldová, Monika - Kuklova, Ivana - Zakoucka, Hana - Šmajs, David
PY  - 2020
TI  - A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results
JF  - Plos one
VL  - 15
IS  - 8
SP  - 1-11
EP  - 1-11
PB  - Public Library of Science
SN  - 19326203
KW  - syphilis treponeme
KW  - PCR detection
UR  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable
L2  - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237949&type=printable
N2  - Syphilis, caused byTreponema pallidumssp.pallidum(TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.
ER  -

VRBOVÁ, Eliška, Lenka MIKALOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Radim STRNADEL, Eliška DASTYCHOVÁ, Martina KOJANOVA, Miluse KREIDLOVA, Daniela VANOUSOVA, Filip ROB, Premysl PROCHAZKA, Alena KRCHŇÁKOVÁ, Vladimír VAŠKŮ, Vladana WOZNICOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Ivana KUKLOVA, Hana ZAKOUCKA and David ŠMAJS. A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results. \textit{Plos one}. San Francisco: Public Library of Science, 2020, vol.~15, No~8, p.~1-11. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0237949.

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