Involvement of the cbb(3)-Type Terminal Oxidase in Growth
Competition of Bacteria, Biofilm Formation, and in Switching
between Denitrification and Aerobic Respiration

J 2020

Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration

KUČERA, Igor a Vojtěch SEDLÁČEK

Základní údaje

Originální název

Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration

Název česky

Účast terminální oxidasy typu cbb(3) při růstové kompetici bakterií, tvorbě biofilmu a přepnutí mezi denitrifikací a aerobní respirací

Autoři

KUČERA, Igor (203 Česká republika, garant, domácí) a Vojtěch SEDLÁČEK (203 Česká republika, domácí)

Vydání

Microorganisms, Basel, MDPI, 2020, 2076-2607

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.128

Kód RIV

RIV/00216224:14310/20:00114320

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/microorganisms8081230

UT WoS

000564643700001

Klíčová slova česky

respirační řetězec; terminální oxidasy; denitrifikace; rozvětvený tok elektronů; biofilm

Klíčová slova anglicky

respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2021 16:51, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.

Návaznosti

GA16-18476S, projekt VaV

Název: Oxidační stres u denitrifikačních baktérií: objasnění funkce zúčastněných proteinů a možných dopadů na životní prostředí

Investor: Grantová agentura ČR, Oxidační stres u denitrifikačních baktérií: objasnění funkce zúčastněných proteinů a možných dopadů na životní prostředí

Citovat

KUČERA, Igor a Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. Microorganisms. Basel: MDPI, 2020, roč. 8, č. 8, s. 1-11. ISSN 2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms8081230.

@article{1679979,
   author = {Kučera, Igor and Sedláček, Vojtěch},
   article_location = {Basel},
   article_number = {8},
   doi = {http://dx.doi.org/10.3390/microorganisms8081230},
   keywords = {respiratory chain; terminal oxidases; denitrification; branched electron flow; biofilm},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration},
   url = {https://doi.org/10.3390/microorganisms8081230},
   volume = {8},
   year = {2020}
}

TY  - JOUR
ID  - 1679979
AU  - Kučera, Igor - Sedláček, Vojtěch
PY  - 2020
TI  - Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration
JF  - Microorganisms
VL  - 8
IS  - 8
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 20762607
KW  - respiratory chain
KW  - terminal oxidases
KW  - denitrification
KW  - branched electron flow
KW  - biofilm
UR  - https://doi.org/10.3390/microorganisms8081230
L2  - https://doi.org/10.3390/microorganisms8081230
N2  - Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa(3)-type and cbb(3)-type cytochrome c oxidases and ba(3)-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb(3)-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa(3)-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb(3) strains, while in strains aa(3) and ba(3) dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb(3)-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.
ER  -

KUČERA, Igor a Vojtěch SEDLÁČEK. Involvement of the cbb(3)-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration. \textit{Microorganisms}. Basel: MDPI, 2020, roč.~8, č.~8, s.~1-11. ISSN~2076-2607. Dostupné z: https://dx.doi.org/10.3390/microorganisms8081230.

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