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@article{1681017,
author = {Vasicek, Ondrej and Hajek, Jan and Bláhová, Lucie and Hrouzek, Pavel and Babica, Pavel and Kubala, Lukas and Sindlerova, Lenka},
article_location = {Amsterdam},
article_number = {June 2020},
doi = {http://dx.doi.org/10.1016/j.hal.2020.101849},
keywords = {Puwainaphycins; Minutissamides; Intestinal epithelium; Tight junction; Inflammation; Cytotoxicity},
language = {eng},
issn = {1568-9883},
journal = {Harmful Algae},
note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.},
title = {Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model},
url = {https://www.sciencedirect.com/science/article/pii/S1568988320301281?via%3Dihub},
volume = {96},
year = {2020}
}
TY - JOUR
ID - 1681017
AU - Vasicek, Ondrej - Hajek, Jan - Bláhová, Lucie - Hrouzek, Pavel - Babica, Pavel - Kubala, Lukas - Sindlerova, Lenka
PY - 2020
TI - Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
JF - Harmful Algae
VL - 96
IS - June 2020
SP - 1-8
EP - 1-8
PB - Elsevier Science
SN - 15689883
N1 - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat.
KW - Puwainaphycins
KW - Minutissamides
KW - Intestinal epithelium
KW - Tight junction
KW - Inflammation
KW - Cytotoxicity
UR - https://www.sciencedirect.com/science/article/pii/S1568988320301281?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S1568988320301281?via%3Dihub
N2 - Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations.
ER -
VASICEK, Ondrej, Jan HAJEK, Lucie BLÁHOVÁ, Pavel HROUZEK, Pavel BABICA, Lukas KUBALA and Lenka SINDLEROVA. Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model. \textit{Harmful Algae}. Amsterdam: Elsevier Science, 2020, vol.~96, June 2020, p.~1-8. ISSN~1568-9883. Available from: https://dx.doi.org/10.1016/j.hal.2020.101849.
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