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@article{1682016, author = {Pešková, Lucie and Jurčíková, Denisa and Váňová, Tereza and Křivánek, Jan and Capandová, Michaela and Šrámková, Zuzana and Šebestíková, Jana and Koloušková, Magdaléna and Kotasová, Hana and Streit, Libor and Bárta, Tomáš}, article_location = {Hoboken}, article_number = {12}, doi = {http://dx.doi.org/10.1002/stem.3272}, keywords = {microRNAs; organoids; pluripotent stem cells; retina}, language = {eng}, issn = {1066-5099}, journal = {Stem Cells}, title = {miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids}, url = {https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272}, volume = {38}, year = {2020} }
TY - JOUR ID - 1682016 AU - Pešková, Lucie - Jurčíková, Denisa - Váňová, Tereza - Křivánek, Jan - Capandová, Michaela - Šrámková, Zuzana - Šebestíková, Jana - Koloušková, Magdaléna - Kotasová, Hana - Streit, Libor - Bárta, Tomáš PY - 2020 TI - miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids JF - Stem Cells VL - 38 IS - 12 SP - 1557-1567 EP - 1557-1567 PB - Wiley-Blackwell SN - 10665099 KW - microRNAs KW - organoids KW - pluripotent stem cells KW - retina UR - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272 L2 - https://stemcellsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/stem.3272 N2 - MicroRNAs (miRNAs), a class of small, noncoding RNA molecules represent important regulators of gene expression. Recent reports have implicated their role in the cell specification process acting as "fine-tuners" to ensure the precise gene expression at the specific stage of cell differentiation. Here, we used retinal organoids differentiated from human pluripotent stem cells (hPSCs) as a model to closely investigate the role of a sensory organ-specific and evolutionary conserved miR-183/96/182 cluster. Using a miRNA tough decoy approach, we inhibited the miR-183/96/182 cluster in hPSCs. Inhibition of the miRNA cluster resulted in an increased expansion of neuroepithelium leading to abnormal "bulged" neural retina in organoids, associated with upregulation of neural-specific and retinal-specific genes. Importantly, we identifiedPAX6, a well-known essential gene in neuroectoderm specification, as a target of the miR-183/96/182 cluster members. Taken together, the miR-183/96/182 cluster not only represents an important regulator ofPAX6expression, but it also plays a crucial role in retinal tissue morphogenesis. ER -
PEŠKOVÁ, Lucie, Denisa JURČÍKOVÁ, Tereza VÁŇOVÁ, Jan KŘIVÁNEK, Michaela CAPANDOVÁ, Zuzana ŠRÁMKOVÁ, Jana ŠEBESTÍKOVÁ, Magdaléna KOLOUŠKOVÁ, Hana KOTASOVÁ, Libor STREIT a Tomáš BÁRTA. miR-183/96/182 cluster is an important morphogenetic factor targetingPAX6expression in differentiating human retinal organoids. \textit{Stem Cells}. Hoboken: Wiley-Blackwell, 2020, roč.~38, č.~12, s.~1557-1567. ISSN~1066-5099. Dostupné z: https://dx.doi.org/10.1002/stem.3272.
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