| JANÁČOVÁ, Lucia, Jakub FAKTOR, Lenka ČÁPKOVÁ, Vendula PÁRALOVÁ, Anna POSPÍŠILOVÁ, Jan PODHOREC, Holger Alexander EBHARDT, Roman HRSTKA, Rudolf NENUTIL, Ruedi AEBERSOLD a Pavel BOUCHAL. SWATH-MS Analysis of FFPE Tissues Identifies Stathmin as a Potential Marker of Endometrial Cancer in Patients Exposed to Tamoxifen. Journal of Proteome Research. Washington, USA: AMER CHEMICAL SOC, 2020, roč. 19, č. 7, s. 2617-2630. ISSN 1535-3893. Dostupné z: https://dx.doi.org/10.1021/acs.jproteome.0c00064. |
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@article{1682959,
author = {Janáčová, Lucia and Faktor, Jakub and Čápková, Lenka and Páralová, Vendula and Pospíšilová, Anna and Podhorec, Jan and Ebhardt, Holger Alexander and Hrstka, Roman and Nenutil, Rudolf and Aebersold, Ruedi and Bouchal, Pavel},
article_location = {Washington, USA},
article_number = {7},
doi = {http://dx.doi.org/10.1021/acs.jproteome.0c00064},
keywords = {endometrial cancer; SWATH-MS; FFPE; calcyphosin; stathmin},
language = {eng},
issn = {1535-3893},
journal = {Journal of Proteome Research},
title = {SWATH-MS Analysis of FFPE Tissues Identifies Stathmin as a Potential Marker of Endometrial Cancer in Patients Exposed to Tamoxifen},
url = {https://doi.org/10.1021/acs.jproteome.0c00064},
volume = {19},
year = {2020}
}
TY - JOUR
ID - 1682959
AU - Janáčová, Lucia - Faktor, Jakub - Čápková, Lenka - Páralová, Vendula - Pospíšilová, Anna - Podhorec, Jan - Ebhardt, Holger Alexander - Hrstka, Roman - Nenutil, Rudolf - Aebersold, Ruedi - Bouchal, Pavel
PY - 2020
TI - SWATH-MS Analysis of FFPE Tissues Identifies Stathmin as a Potential Marker of Endometrial Cancer in Patients Exposed to Tamoxifen
JF - Journal of Proteome Research
VL - 19
IS - 7
SP - 2617-2630
EP - 2617-2630
PB - AMER CHEMICAL SOC
SN - 15353893
KW - endometrial cancer
KW - SWATH-MS
KW - FFPE
KW - calcyphosin
KW - stathmin
UR - https://doi.org/10.1021/acs.jproteome.0c00064
L2 - https://doi.org/10.1021/acs.jproteome.0c00064
N2 - A specific form of endometrial cancer (EC) can develop in breast cancer patients previously treated with tamoxifen (ET), an antagonist of estrogen receptor (ER) that inhibits proliferation of ER positive breast cancer. ET tumors have a different phenotype than endometrial tumors, which typically develop de novo without previous exposure to tamoxifen (EN). Here we aimed to identify specific protein markers that could serve as specific molecular targets in either phenotype. A set of total 45 formalin-fixed paraffin-embedded (FFPE) endometrial tumor tissues and adjacent myometrium tissue samples were analyzed using LC-MS/MS in SWATH-MS mode. We found that calcyphosin (CAPS) levels were elevated in EN tumors compared to ET tumors. The higher CAPS level in EC tissue invading to myometrium supports its relationship to EC aggressiveness. Further, stathmin (STMN1) levels were found significantly elevated in ET versus EN tumors and significantly associated with patient survival. This finding connects elevated levels of this cell cycle regulating, proliferation-associated protein with tamoxifen exposure. In summary, using SWATH-MS we show that CAPS and STMN1 should be recognized as clinicopathologically different EC markers of which STMN1 is specifically connected with a previous tamoxifen exposition.
ER -
JANÁČOVÁ, Lucia, Jakub FAKTOR, Lenka ČÁPKOVÁ, Vendula PÁRALOVÁ, Anna POSPÍŠILOVÁ, Jan PODHOREC, Holger Alexander EBHARDT, Roman HRSTKA, Rudolf NENUTIL, Ruedi AEBERSOLD a Pavel BOUCHAL. SWATH-MS Analysis of FFPE Tissues Identifies Stathmin as a Potential Marker of Endometrial Cancer in Patients Exposed to Tamoxifen. \textit{Journal of Proteome Research}. Washington, USA: AMER CHEMICAL SOC, 2020, roč.~19, č.~7, s.~2617-2630. ISSN~1535-3893. Dostupné z: https://dx.doi.org/10.1021/acs.jproteome.0c00064.
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