Analysis of Bacteriophage-Host Interaction by Raman Tweezers

J 2020

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

PILAT, Zdenek, Alexandr JONAS, Jana PILATOVA, Tereza KLEMENTOVA, Silvie BERNATOVA et. al.

Základní údaje

Originální název

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Autoři

PILAT, Zdenek (203 Česká republika, garant), Alexandr JONAS (203 Česká republika), Jana PILATOVA (203 Česká republika), Tereza KLEMENTOVA (203 Česká republika), Silvie BERNATOVA (203 Česká republika), Martin SILER (203 Česká republika), Tadeas MANKA (203 Česká republika), Martin KIZOVSKY (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, domácí), Roman PANTŮČEK (203 Česká republika, domácí), Ute NEUGEBAUER (276 Německo), Ota SAMEK (203 Česká republika) a Pavel ZEMANEK (203 Česká republika)

Vydání

Analytical chemistry, Washington, American Chemical Society, 2020, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.986

Kód RIV

RIV/00216224:14110/20:00114355

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1021/acs.analchem.0c01963

UT WoS

000572832900029

Klíčová slova anglicky

Infectious diseases; Viruses; Bioinorganic chemistry; Genetics; Raman spectroscopy

Štítky

14110113, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 1. 2021 15:01, Mgr. Tereza Miškechová

Anotace

V originále

Bacteriophages, or "phages" for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.

Návaznosti

GA18-13064S, projekt VaV

Název: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Grantová agentura ČR, Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

MUNI/A/1237/2019, interní kód MU

Název: Diagnostika nozokomiálních infekcí a možnosti jejich prevence a terapie (Akronym: DNIMJPT)

Investor: Masarykova univerzita, Diagnostika nozokomiálních infekcí a možnosti jejich prevence a terapie, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

Citovat

PILAT, Zdenek, Alexandr JONAS, Jana PILATOVA, Tereza KLEMENTOVA, Silvie BERNATOVA, Martin SILER, Tadeas MANKA, Martin KIZOVSKY, Filip RŮŽIČKA, Roman PANTŮČEK, Ute NEUGEBAUER, Ota SAMEK a Pavel ZEMANEK. Analysis of Bacteriophage-Host Interaction by Raman Tweezers. Analytical chemistry. Washington: American Chemical Society, 2020, roč. 92, č. 18, s. 12304-12311. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.0c01963.

@article{1684416,
   author = {Pilat, Zdenek and Jonas, Alexandr and Pilatova, Jana and Klementova, Tereza and Bernatova, Silvie and Siler, Martin and Manka, Tadeas and Kizovsky, Martin and Růžička, Filip and Pantůček, Roman and Neugebauer, Ute and Samek, Ota and Zemanek, Pavel},
   article_location = {Washington},
   article_number = {18},
   doi = {http://dx.doi.org/10.1021/acs.analchem.0c01963},
   keywords = {Infectious diseases; Viruses; Bioinorganic chemistry; Genetics; Raman spectroscopy},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {Analysis of Bacteriophage-Host Interaction by Raman Tweezers},
   url = {https://doi.org/10.1021/acs.analchem.0c01963},
   volume = {92},
   year = {2020}
}

TY  - JOUR
ID  - 1684416
AU  - Pilat, Zdenek - Jonas, Alexandr - Pilatova, Jana - Klementova, Tereza - Bernatova, Silvie - Siler, Martin - Manka, Tadeas - Kizovsky, Martin - Růžička, Filip - Pantůček, Roman - Neugebauer, Ute - Samek, Ota - Zemanek, Pavel
PY  - 2020
TI  - Analysis of Bacteriophage-Host Interaction by Raman Tweezers
JF  - Analytical chemistry
VL  - 92
IS  - 18
SP  - 12304-12311
EP  - 12304-12311
PB  - American Chemical Society
SN  - 00032700
KW  - Infectious diseases
KW  - Viruses
KW  - Bioinorganic chemistry
KW  - Genetics
KW  - Raman spectroscopy
UR  - https://doi.org/10.1021/acs.analchem.0c01963
L2  - https://doi.org/10.1021/acs.analchem.0c01963
N2  - Bacteriophages, or "phages" for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
ER  -

PILAT, Zdenek, Alexandr JONAS, Jana PILATOVA, Tereza KLEMENTOVA, Silvie BERNATOVA, Martin SILER, Tadeas MANKA, Martin KIZOVSKY, Filip RŮŽIČKA, Roman PANTŮČEK, Ute NEUGEBAUER, Ota SAMEK a Pavel ZEMANEK. Analysis of Bacteriophage-Host Interaction by Raman Tweezers. \textit{Analytical chemistry}. Washington: American Chemical Society, 2020, roč.~92, č.~18, s.~12304-12311. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.0c01963.

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