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@article{1685061,
author = {Peltomaa, Riikka and Farka, Zdeněk and Mickert, Matthias Jürgen and Brandmeier, Julian and Pastucha, Matěj and Hlaváček, Antonín and MartínezandOrts, Mónica and Canales, Ángeles and Skládal, Petr and BenitoandPeña, Elena and MorenoandBondi, María C. and Gorris, HansandHeiner},
article_location = {Oxford},
article_number = {December 2020},
doi = {http://dx.doi.org/10.1016/j.bios.2020.112683},
keywords = {Zearalenone; Upconversion nanoparticle; Peptide mimetic; Immunosensing; Surface plasmon resonance; Food safety},
language = {eng},
issn = {0956-5663},
journal = {Biosensors and Bioelectronics},
title = {Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone},
url = {https://www.sciencedirect.com/science/article/pii/S0956566320306722},
volume = {170},
year = {2020}
}
TY - JOUR
ID - 1685061
AU - Peltomaa, Riikka - Farka, Zdeněk - Mickert, Matthias Jürgen - Brandmeier, Julian - Pastucha, Matěj - Hlaváček, Antonín - Martínez-Orts, Mónica - Canales, Ángeles - Skládal, Petr - Benito-Peña, Elena - Moreno-Bondi, María C. - Gorris, Hans-Heiner
PY - 2020
TI - Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone
JF - Biosensors and Bioelectronics
VL - 170
IS - December 2020
SP - 1-9
EP - 1-9
PB - Elsevier B.V.
SN - 09565663
KW - Zearalenone
KW - Upconversion nanoparticle
KW - Peptide mimetic
KW - Immunosensing
KW - Surface plasmon resonance
KW - Food safety
UR - https://www.sciencedirect.com/science/article/pii/S0956566320306722
L2 - https://www.sciencedirect.com/science/article/pii/S0956566320306722
N2 - Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL-1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (alpha- and beta-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples.
ER -
PELTOMAA, Riikka, Zdeněk FARKA, Matthias Jürgen MICKERT, Julian BRANDMEIER, Matěj PASTUCHA, Antonín HLAVÁČEK, Mónica MARTÍNEZ-ORTS, Ángeles CANALES, Petr SKLÁDAL, Elena BENITO-PEÑA, María C. MORENO-BONDI a Hans-Heiner GORRIS. Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone. \textit{Biosensors and Bioelectronics}. Oxford: Elsevier B.V., 2020, roč.~170, December 2020, s.~1-9. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2020.112683.
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