J 2020

Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

SMRČKA, Filip and Přemysl LUBAL

Basic information

Original name

Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection

Authors

SMRČKA, Filip (203 Czech Republic, belonging to the institution) and Přemysl LUBAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Molecules, Basel, MDPI, 2020, 1420-3049

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.411

RIV identification code

RIV/00216224:14310/20:00116793

Organization unit

Faculty of Science

DOI

UT WoS

000580745000001

Keywords (in Czech)

Ln(III) komplexy; makrocyklické ligandy; Ln(III) luminescence ve VIS/NIR oblasti; antenový ligand; NAD(P)H stanovení; enzymová sonda

Keywords in English

Ln(III) complexes; macrocyclic ligands; Ln(III) luminescence in VIS/NIR range; antenna ligand; NAD(P)H determination; enzyme probe

Tags

Tags

International impact, Reviewed
Změněno: 4/12/2020 15:27, Mgr. Marie Šípková, DiS.

Abstract

V originále

Ln(III) complexes of macrocyclic ligands are used in medicinal chemistry, for example as contrast agents in MRI or radiopharmaceutical compounds, and in diagnostics using fluorescence imaging. This paper is devoted to a spectroscopic study of Ln(III) ternary complexes consisting of macrocyclic heptadentate DO3A and bidentate 3-isoquinolinate (IQCA) ligands. IQCA serves as an efficient antenna ligand, leading to a higher quantum yield and Stokes shift (250–350 nm for Eu, Tb, Sm, Dy in VIS region, 550–650 nm for Yb, Nd in NIR region). The shielding-quenching effect of NAD(P)H on the luminescence of the Ln(III) ternary complexes was investigated in detail and this phenomenon was utilized for the analytical determination of this compound. This general approach was verified through an enzymatic reaction during which the course of ethanol transformation catalyzed by alcohol-dehydrogenase (ADH) was followed by luminescence spectroscopy. This method can be utilized for selective and sensitive determination of ethanol concentration and/or ADH enzyme activity. This new analytical method can also be used for other enzyme systems coupled with NAD(P)H/NAD(P)+ redox pairs.

Links

LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTC20044, research and development project
Name: Thermodynamic and kinetic investigations of metal complexes of polydentate ligands for biomedical, analytical and industrial applications (Acronym: NECTAR_COST)
Investor: Ministry of Education, Youth and Sports of the CR, INTER-COST
MUNI/A/1424/2019, interní kód MU
Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2020
Investor: Masaryk University, Category A
692298, interní kód MU
Name: MEDGENET - Medical genomics and epigenomics network (Acronym: MEDGENET)
Investor: European Union, Spreading excellence and widening participation