Cell type specific adhesion to surfaces functionalised by amine
plasma polymer

J 2020

Cell type specific adhesion to surfaces functionalised by amine plasma polymer

ČERNOCHOVÁ, Petra, Lucie BLAHOVÁ, Jiřina MEDALOVÁ, David NEČAS, Miroslav MICHLÍČEK et. al.

Základní údaje

Originální název

Cell type specific adhesion to surfaces functionalised by amine plasma polymer

Autoři

ČERNOCHOVÁ, Petra (203 Česká republika, domácí), Lucie BLAHOVÁ (203 Česká republika, domácí), Jiřina MEDALOVÁ (203 Česká republika, domácí), David NEČAS (203 Česká republika, domácí), Miroslav MICHLÍČEK (203 Česká republika, domácí), Preeti KAUSHIK (356 Indie, domácí), Jan PŘIBYL (203 Česká republika, domácí), Jana BARTOŠÍKOVÁ (203 Česká republika, domácí), Anton MANAKHOV (643 Rusko, domácí), Lucie BAČÁKOVÁ (203 Česká republika) a Lenka ZAJÍČKOVÁ (203 Česká republika, garant, domácí)

Vydání

Scientific Reports, London, Nature Publishing Group, 2020, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10305 Fluids and plasma physics

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.379

Kód RIV

RIV/00216224:14310/20:00114429

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/s41598-020-65889-y

UT WoS

000543956100001

Klíčová slova anglicky

Biomaterials - cells; Cell adhesion

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 11:47, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.

Návaznosti

GA18-12774S, projekt VaV

Název: Plazmové polymery připravené na nanovlákenných membránách pro inženýrství cévní tkáně

Investor: Grantová agentura ČR, Plazmové polymery připravené na nanovlákenných membránách pro inženýrství cévní tkáně

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

ČERNOCHOVÁ, Petra, Lucie BLAHOVÁ, Jiřina MEDALOVÁ, David NEČAS, Miroslav MICHLÍČEK, Preeti KAUSHIK, Jan PŘIBYL, Jana BARTOŠÍKOVÁ, Anton MANAKHOV, Lucie BAČÁKOVÁ a Lenka ZAJÍČKOVÁ. Cell type specific adhesion to surfaces functionalised by amine plasma polymer. Scientific Reports. London: Nature Publishing Group, 2020, roč. 10, č. 1, s. 1-14. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-65889-y.

@article{1689019,
   author = {Černochová, Petra and Blahová, Lucie and Medalová, Jiřina and Nečas, David and Michlíček, Miroslav and Kaushik, Preeti and Přibyl, Jan and Bartošíková, Jana and Manakhov, Anton and Bačáková, Lucie and Zajíčková, Lenka},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-65889-y},
   keywords = {Biomaterials - cells; Cell adhesion},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   note = {doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024},
   title = {Cell type specific adhesion to surfaces functionalised by amine plasma polymer},
   url = {https://www.nature.com/articles/s41598-020-65889-y},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1689019
AU  - Černochová, Petra - Blahová, Lucie - Medalová, Jiřina - Nečas, David - Michlíček, Miroslav - Kaushik, Preeti - Přibyl, Jan - Bartošíková, Jana - Manakhov, Anton - Bačáková, Lucie - Zajíčková, Lenka
PY  - 2020
TI  - Cell type specific adhesion to surfaces functionalised by amine plasma polymer
JF  - Scientific Reports
VL  - 10
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - Nature Publishing Group
SN  - 20452322
N1  - doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024
KW  - Biomaterials - cells
KW  - Cell adhesion
UR  - https://www.nature.com/articles/s41598-020-65889-y
L2  - https://www.nature.com/articles/s41598-020-65889-y
N2  - Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.
ER  -

ČERNOCHOVÁ, Petra, Lucie BLAHOVÁ, Jiřina MEDALOVÁ, David NEČAS, Miroslav MICHLÍČEK, Preeti KAUSHIK, Jan PŘIBYL, Jana BARTOŠÍKOVÁ, Anton MANAKHOV, Lucie BAČÁKOVÁ a Lenka ZAJÍČKOVÁ. Cell type specific adhesion to surfaces functionalised by amine plasma polymer. \textit{Scientific Reports}. London: Nature Publishing Group, 2020, roč.~10, č.~1, s.~1-14. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-020-65889-y.

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