Near-infrared Polymethine Dyes for Targeted Biomarker Detection

a 2020

Near-infrared Polymethine Dyes for Targeted Biomarker Detection

PASTUCHA, Matěj, Martin POTRUSIL, Zdeněk FARKA a Peter ŠEBEJ

Základní údaje

Originální název

Near-infrared Polymethine Dyes for Targeted Biomarker Detection

Autoři

PASTUCHA, Matěj, Martin POTRUSIL, Zdeněk FARKA a Peter ŠEBEJ

Vydání

XXth Interdisciplinary Meeting of Young Researchers and Students in the Field of Chemistry, Biochemistry, Molecular Biology, and Biomaterials, 2020

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

ISSN

Změněno: 9. 11. 2020 11:34, Mgr. Matěj Pastucha, Ph.D.

Anotace

V originále

Fluorescent dyes have a wide scope of use in life-sciences and medicine, most commonly as imaging agents, either alone, or in connection with antibodies in immunofluorescence experiments. Biological tissues absorb visible light and a tissue-transparent window (TTW) in the near-infrared (NIR) region is preferred for imaging purposes. This is particularly true in the fluorescence image-guided surgery. Polymethine dyes are good candidates for applications in the TTW and their properties can be tuned by changes in the polymethine chain length and substituents. We chose the indocyanine green (ICG) as an initial model as it is the only NIR dye certified by the FDA for use in clinical medicine. It is usually used as a dye with very limited selectivity, but when conjugated to an antibody, it can also be used for specific visualization of a biomarker of interest. We chose the carbohydrate antigen (CA) 19-9 as a promising target because it is already successfully used in the diagnosis and monitoring of treatment outcome in patients with pancreatic cancer. First, we labeled the mouse anti CA 19-9 antibody with the ICG NHS ester. The conjugate was successfully tested in a microtiter plate-based fluorescence immunoassay. Testing of the conjugate for detection of CA 19-9 in cell lines and tissue sections follows. We aim to develop a platform for assessing the performance of the newly synthesized polymethine fluorescent labels in complex biological systems.

Návaznosti

GJ20-30004Y, projekt VaV

Název: Nové strukturní motivy pro rozšíření možností lineárních polymethinových barviv

Investor: Grantová agentura ČR, Nové strukturní motivy pro rozšíření možností lineárních polymethinových barviv

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

PASTUCHA, Matěj, Martin POTRUSIL, Zdeněk FARKA a Peter ŠEBEJ. Near-infrared Polymethine Dyes for Targeted Biomarker Detection. In XXth Interdisciplinary Meeting of Young Researchers and Students in the Field of Chemistry, Biochemistry, Molecular Biology, and Biomaterials. 2020. ISSN 2336-7202.

@proceedings{1690956,
   author = {Pastucha, Matěj and Potrusil, Martin and Farka, Zdeněk and Šebej, Peter},
   booktitle = {XXth Interdisciplinary Meeting of Young Researchers and Students in the Field of Chemistry, Biochemistry, Molecular Biology, and Biomaterials},
   language = {eng},
   title = {Near-infrared Polymethine Dyes for Targeted Biomarker Detection},
   url = {http://www.ccsss.cz/index.php/ccsss/issue/viewIssue/27/39},
   year = {2020}
}

TY  - CONF
ID  - 1690956
AU  - Pastucha, Matěj - Potrusil, Martin - Farka, Zdeněk - Šebej, Peter
PY  - 2020
TI  - Near-infrared Polymethine Dyes for Targeted Biomarker Detection
UR  - http://www.ccsss.cz/index.php/ccsss/issue/viewIssue/27/39
N2  - Fluorescent dyes have a wide scope of use in life-sciences and medicine, most commonly as imaging agents, either alone, or in connection with antibodies in immunofluorescence experiments. Biological tissues absorb visible light and a tissue-transparent window (TTW) in the near-infrared (NIR) region is preferred for imaging purposes. This is particularly true in the fluorescence image-guided surgery. Polymethine dyes are good candidates for applications in the TTW and their properties can be tuned by changes in the polymethine chain length and substituents. We chose the indocyanine green (ICG) as an initial model as it is the only NIR dye certified by the FDA for use in clinical medicine. It is usually used as a dye with very limited selectivity, but when conjugated to an antibody, it can also be used for specific visualization of a biomarker of interest. We chose the carbohydrate antigen (CA) 19-9 as a promising target because it is already successfully used in the diagnosis and monitoring of treatment outcome in patients with pancreatic cancer. First, we labeled the mouse anti CA 19-9 antibody with the ICG NHS ester. The conjugate was successfully tested in a microtiter plate-based fluorescence immunoassay. Testing of the conjugate for detection of CA 19-9 in cell lines and tissue sections follows. We aim to develop a platform for assessing the performance of the newly synthesized polymethine fluorescent labels in complex biological systems.
ER  -

PASTUCHA, Matěj, Martin POTRUSIL, Zdeněk FARKA a Peter ŠEBEJ. Near-infrared Polymethine Dyes for Targeted Biomarker Detection. In \textit{XXth Interdisciplinary Meeting of Young Researchers and Students in the Field of Chemistry, Biochemistry, Molecular Biology, and Biomaterials}. 2020. ISSN~2336-7202.

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