Versatile Bioconjugation Strategies of PEG-Modified
Upconversion Nanoparticles for Bioanalytical Applications

J 2020

Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications

KOSTIV, Uliana, Zdeněk FARKA, Matthias Jürgen MICKERT, Hans-Heiner GORRIS, Nadiia VELYCHKIVSKA et. al.

Základní údaje

Originální název

Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications

Autoři

KOSTIV, Uliana (804 Ukrajina), Zdeněk FARKA (203 Česká republika, garant, domácí), Matthias Jürgen MICKERT (276 Německo), Hans-Heiner GORRIS (276 Německo), Nadiia VELYCHKIVSKA (203 Česká republika), Ognen POP-GEORGIEVSKI (203 Česká republika), Matěj PASTUCHA (203 Česká republika, domácí), Eliška ODSTRČILÍKOVÁ (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Daniel HORÁK (203 Česká republika)

Vydání

Biomacromolecules, Washington, DC, American Chemical Society, 2020, 1525-7797

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.988

Kód RIV

RIV/00216224:14310/20:00116881

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/acs.biomac.0c00459

UT WoS

000592221000010

Klíčová slova anglicky

photon-upconversion nanoparticle; UCNP; bioconjugation; poly(ethylene glycol); click chemistry; upconversion-linked immunosorbent assay; ULISA; human serum albumin

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 1. 2021 10:12, Mgr. Marie Šípková, DiS.

Anotace

V originále

Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

LTAB19011, projekt VaV

Název: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel, INTER-ACTION

Citovat

KOSTIV, Uliana, Zdeněk FARKA, Matthias Jürgen MICKERT, Hans-Heiner GORRIS, Nadiia VELYCHKIVSKA, Ognen POP-GEORGIEVSKI, Matěj PASTUCHA, Eliška ODSTRČILÍKOVÁ, Petr SKLÁDAL a Daniel HORÁK. Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications. Biomacromolecules. Washington, DC: American Chemical Society, 2020, roč. 21, č. 11, s. 4502-4513. ISSN 1525-7797. Dostupné z: https://dx.doi.org/10.1021/acs.biomac.0c00459.

@article{1690977,
   author = {Kostiv, Uliana and Farka, Zdeněk and Mickert, Matthias Jürgen and Gorris, HansandHeiner and Velychkivska, Nadiia and PopandGeorgievski, Ognen and Pastucha, Matěj and Odstrčilíková, Eliška and Skládal, Petr and Horák, Daniel},
   article_location = {Washington, DC},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.biomac.0c00459},
   keywords = {photon-upconversion nanoparticle; UCNP; bioconjugation; poly(ethylene glycol); click chemistry; upconversion-linked immunosorbent assay; ULISA; human serum albumin},
   language = {eng},
   issn = {1525-7797},
   journal = {Biomacromolecules},
   title = {Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications},
   url = {https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1690977
AU  - Kostiv, Uliana - Farka, Zdeněk - Mickert, Matthias Jürgen - Gorris, Hans-Heiner - Velychkivska, Nadiia - Pop-Georgievski, Ognen - Pastucha, Matěj - Odstrčilíková, Eliška - Skládal, Petr - Horák, Daniel
PY  - 2020
TI  - Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications
JF  - Biomacromolecules
VL  - 21
IS  - 11
SP  - 4502-4513
EP  - 4502-4513
PB  - American Chemical Society
SN  - 15257797
KW  - photon-upconversion nanoparticle
KW  - UCNP
KW  - bioconjugation
KW  - poly(ethylene glycol)
KW  - click chemistry
KW  - upconversion-linked immunosorbent assay
KW  - ULISA
KW  - human serum albumin
UR  - https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459
L2  - https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459
N2  - Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).
ER  -

KOSTIV, Uliana, Zdeněk FARKA, Matthias Jürgen MICKERT, Hans-Heiner GORRIS, Nadiia VELYCHKIVSKA, Ognen POP-GEORGIEVSKI, Matěj PASTUCHA, Eliška ODSTRČILÍKOVÁ, Petr SKLÁDAL a Daniel HORÁK. Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications. \textit{Biomacromolecules}. Washington, DC: American Chemical Society, 2020, roč.~21, č.~11, s.~4502-4513. ISSN~1525-7797. Dostupné z: https://dx.doi.org/10.1021/acs.biomac.0c00459.

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