Další formáty:
BibTeX
LaTeX
RIS
@article{1692064,
author = {Kula, Roman and Bébarová, Markéta and Matejovič, Peter and Šimurda, Jiří and Pásek, Michal},
article_location = {OXFORD},
article_number = {NOV 2020},
doi = {http://dx.doi.org/10.1016/j.pbiomolbio.2019.11.011},
keywords = {Ionic current; Current density; Current-capacitance correlation; Current-capacitance proportionality; Rat cardiomyocyte; Normalisation by ratio},
language = {eng},
issn = {0079-6107},
journal = {PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY},
title = {Current density as routine parameter for description of ionic membrane current: is it always the best option?},
url = {https://www.sciencedirect.com/science/article/pii/S0079610719301452?via%3Dihub},
volume = {157},
year = {2020}
}
TY - JOUR
ID - 1692064
AU - Kula, Roman - Bébarová, Markéta - Matejovič, Peter - Šimurda, Jiří - Pásek, Michal
PY - 2020
TI - Current density as routine parameter for description of ionic membrane current: is it always the best option?
JF - PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
VL - 157
IS - NOV 2020
SP - 24-32
EP - 24-32
PB - PERGAMON-ELSEVIER SCIENCE LTD
SN - 00796107
KW - Ionic current
KW - Current density
KW - Current-capacitance correlation
KW - Current-capacitance proportionality
KW - Rat cardiomyocyte
KW - Normalisation by ratio
UR - https://www.sciencedirect.com/science/article/pii/S0079610719301452?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0079610719301452?via%3Dihub
N2 - The current density (J) is a parameter routinely used to characterize individual ionic membrane currents. Its evaluation is based on the presumption that the magnitude of whole-cell ionic membrane current (I) is directly proportional to the cell membrane capacitance (C), i.e. I positively and strongly correlates with C and the regression line describing I-C relation intersects the y-axis close to the origin of coordinates. We aimed to prove the presumption in several examples and find whether the conversion of I to J could be always beneficial. I-C relation was analysed in several potassium currents, measured in rat atrial myocytes (in inward rectifier currents, I-K1, and both the constitutively active and acetylcholine-induced components of acetylcholine-sensitive current, I-K(Ach)CONST and I-K(Ach)ACH), and in rat ventricular myocytes (transient outward current I-to). I-C correlation was estimated by the Pearson coefficient (r). A coefficient (k) was newly suggested describing deviation of the regression intercept from zero in currents with considerable r value. Based on mathematical simulations, I was satisfactorily proportional to C when r >= 0.6 and k <= 0.2 which was fulfilled in I-K1 and I-K(Ach)ACH (r = 0.84, k = 0.20, and r = 0.61, k = 0.06, respectively). IeC correlation was significantly positive, but weak in I-K(Ach)CONST (r = 0.42), and virtually missing in Ito (r = 0.04). The impaired I-C proportionality in I-K(Ach)CONST and Ito likely reflects heterogeneity of the channel expression. We conclude that the conversion of I to J should be avoided when I-C proportionality is absent. Otherwise, serious misinterpretation of data may arise. (C) 2019 Elsevier Ltd. All rights reserved.
ER -
KULA, Roman, Markéta BÉBAROVÁ, Peter MATEJOVIČ, Jiří ŠIMURDA a Michal PÁSEK. Current density as routine parameter for description of ionic membrane current: is it always the best option? \textit{PROGRESS IN BIOPHYSICS \&{}amp; MOLECULAR BIOLOGY}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2020, roč.~157, NOV 2020, s.~24-32. ISSN~0079-6107. Dostupné z: https://dx.doi.org/10.1016/j.pbiomolbio.2019.11.011.
|
