Current density as routine parameter for description of ionic
membrane current: is it always the best option?

J 2020

Current density as routine parameter for description of ionic membrane current: is it always the best option?

KULA, Roman, Markéta BÉBAROVÁ, Peter MATEJOVIČ, Jiří ŠIMURDA, Michal PÁSEK et. al.

Základní údaje

Originální název

Current density as routine parameter for description of ionic membrane current: is it always the best option?

Autoři

KULA, Roman (203 Česká republika, domácí), Markéta BÉBAROVÁ (203 Česká republika, garant, domácí), Peter MATEJOVIČ (203 Česká republika, domácí), Jiří ŠIMURDA (203 Česká republika, domácí) a Michal PÁSEK (203 Česká republika, domácí)

Vydání

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2020, 0079-6107

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.667

Kód RIV

RIV/00216224:14110/20:00118627

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.pbiomolbio.2019.11.011

UT WoS

000582745400005

Klíčová slova anglicky

Ionic current; Current density; Current-capacitance correlation; Current-capacitance proportionality; Rat cardiomyocyte; Normalisation by ratio

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 5. 2021 14:14, Mgr. Tereza Miškechová

Anotace

V originále

The current density (J) is a parameter routinely used to characterize individual ionic membrane currents. Its evaluation is based on the presumption that the magnitude of whole-cell ionic membrane current (I) is directly proportional to the cell membrane capacitance (C), i.e. I positively and strongly correlates with C and the regression line describing I-C relation intersects the y-axis close to the origin of coordinates. We aimed to prove the presumption in several examples and find whether the conversion of I to J could be always beneficial. I-C relation was analysed in several potassium currents, measured in rat atrial myocytes (in inward rectifier currents, I-K1, and both the constitutively active and acetylcholine-induced components of acetylcholine-sensitive current, I-K(Ach)CONST and I-K(Ach)ACH), and in rat ventricular myocytes (transient outward current I-to). I-C correlation was estimated by the Pearson coefficient (r). A coefficient (k) was newly suggested describing deviation of the regression intercept from zero in currents with considerable r value. Based on mathematical simulations, I was satisfactorily proportional to C when r >= 0.6 and k <= 0.2 which was fulfilled in I-K1 and I-K(Ach)ACH (r = 0.84, k = 0.20, and r = 0.61, k = 0.06, respectively). IeC correlation was significantly positive, but weak in I-K(Ach)CONST (r = 0.42), and virtually missing in Ito (r = 0.04). The impaired I-C proportionality in I-K(Ach)CONST and Ito likely reflects heterogeneity of the channel expression. We conclude that the conversion of I to J should be avoided when I-C proportionality is absent. Otherwise, serious misinterpretation of data may arise. (C) 2019 Elsevier Ltd. All rights reserved.

Návaznosti

NV16-30571A, projekt VaV

Název: Klinický význam a elektrofyziologické zhodnocení mutace c.926C>T genu KCNQ1 (p.T309I) jako možné „founder mutation“ syndromu dlouhého intervalu QT

Citovat

KULA, Roman, Markéta BÉBAROVÁ, Peter MATEJOVIČ, Jiří ŠIMURDA a Michal PÁSEK. Current density as routine parameter for description of ionic membrane current: is it always the best option? PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2020, roč. 157, NOV 2020, s. 24-32. ISSN 0079-6107. Dostupné z: https://dx.doi.org/10.1016/j.pbiomolbio.2019.11.011.

@article{1692064,
   author = {Kula, Roman and Bébarová, Markéta and Matejovič, Peter and Šimurda, Jiří and Pásek, Michal},
   article_location = {OXFORD},
   article_number = {NOV 2020},
   doi = {http://dx.doi.org/10.1016/j.pbiomolbio.2019.11.011},
   keywords = {Ionic current; Current density; Current-capacitance correlation; Current-capacitance proportionality; Rat cardiomyocyte; Normalisation by ratio},
   language = {eng},
   issn = {0079-6107},
   journal = {PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY},
   title = {Current density as routine parameter for description of ionic membrane current: is it always the best option?},
   url = {https://www.sciencedirect.com/science/article/pii/S0079610719301452?via%3Dihub},
   volume = {157},
   year = {2020}
}

TY  - JOUR
ID  - 1692064
AU  - Kula, Roman - Bébarová, Markéta - Matejovič, Peter - Šimurda, Jiří - Pásek, Michal
PY  - 2020
TI  - Current density as routine parameter for description of ionic membrane current: is it always the best option?
JF  - PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
VL  - 157
IS  - NOV 2020
SP  - 24-32
EP  - 24-32
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00796107
KW  - Ionic current
KW  - Current density
KW  - Current-capacitance correlation
KW  - Current-capacitance proportionality
KW  - Rat cardiomyocyte
KW  - Normalisation by ratio
UR  - https://www.sciencedirect.com/science/article/pii/S0079610719301452?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0079610719301452?via%3Dihub
N2  - The current density (J) is a parameter routinely used to characterize individual ionic membrane currents. Its evaluation is based on the presumption that the magnitude of whole-cell ionic membrane current (I) is directly proportional to the cell membrane capacitance (C), i.e. I positively and strongly correlates with C and the regression line describing I-C relation intersects the y-axis close to the origin of coordinates. We aimed to prove the presumption in several examples and find whether the conversion of I to J could be always beneficial. I-C relation was analysed in several potassium currents, measured in rat atrial myocytes (in inward rectifier currents, I-K1, and both the constitutively active and acetylcholine-induced components of acetylcholine-sensitive current, I-K(Ach)CONST and I-K(Ach)ACH), and in rat ventricular myocytes (transient outward current I-to). I-C correlation was estimated by the Pearson coefficient (r). A coefficient (k) was newly suggested describing deviation of the regression intercept from zero in currents with considerable r value. Based on mathematical simulations, I was satisfactorily proportional to C when r >= 0.6 and k <= 0.2 which was fulfilled in I-K1 and I-K(Ach)ACH (r = 0.84, k = 0.20, and r = 0.61, k = 0.06, respectively). IeC correlation was significantly positive, but weak in I-K(Ach)CONST (r = 0.42), and virtually missing in Ito (r = 0.04). The impaired I-C proportionality in I-K(Ach)CONST and Ito likely reflects heterogeneity of the channel expression. We conclude that the conversion of I to J should be avoided when I-C proportionality is absent. Otherwise, serious misinterpretation of data may arise. (C) 2019 Elsevier Ltd. All rights reserved.
ER  -

KULA, Roman, Markéta BÉBAROVÁ, Peter MATEJOVIČ, Jiří ŠIMURDA a Michal PÁSEK. Current density as routine parameter for description of ionic membrane current: is it always the best option? \textit{PROGRESS IN BIOPHYSICS \&{}amp; MOLECULAR BIOLOGY}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2020, roč.~157, NOV 2020, s.~24-32. ISSN~0079-6107. Dostupné z: https://dx.doi.org/10.1016/j.pbiomolbio.2019.11.011.

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