Další formáty:
BibTeX
LaTeX
RIS
@article{1697996,
author = {Vašíček, Ondřej and Lojek, Antonín and Číž, Milan},
article_location = {Dordrecht},
article_number = {1},
doi = {http://dx.doi.org/10.1007/s13105-019-00714-3},
keywords = {Serotonin; N-acetylserotonin; Melatonin; RAW264; 7 macrophages; Reactive oxygen species; Nitric oxide; Cytokines},
language = {eng},
issn = {1138-7548},
journal = {Journal of Physiology and Biochemistry},
title = {Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation},
url = {https://doi.org/10.1007/s13105-019-00714-3},
volume = {76},
year = {2020}
}
TY - JOUR
ID - 1697996
AU - Vašíček, Ondřej - Lojek, Antonín - Číž, Milan
PY - 2020
TI - Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation
JF - Journal of Physiology and Biochemistry
VL - 76
IS - 1
SP - 49-60
EP - 49-60
PB - Springer
SN - 11387548
KW - Serotonin
KW - N-acetylserotonin
KW - Melatonin
KW - RAW264
KW - 7 macrophages
KW - Reactive oxygen species
KW - Nitric oxide
KW - Cytokines
UR - https://doi.org/10.1007/s13105-019-00714-3
L2 - https://doi.org/10.1007/s13105-019-00714-3
N2 - In this study, we focused on comparing the effects of serotonin and its metabolites on the functions of RAW264.7 cells (emphasis on oxidative burst and production of nitric oxide and cytokines), thereby expanding the scope of existing knowledge with advent of novel findings in this field. Changes in production of reactive oxygen species (ROS) by RAW264.7 cells after treatment with serotonin, N-acetylserotonin and melatonin were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all respective compounds were measured using TRAP and amperometrical method. Nitric oxide (NO) production was measured by Griess reagent and inducible NO synthase (iNOS) expression by Western blot. Cytokine production was assessed using the Mouse Cytokine Panel A Array kit and ELISA. We showed that all tested compounds were able to reduce oxidative stress, as well as inhibit production of inflammatory cytokines by macrophages. Of the tested compounds, serotonin and N-acetylserotonin were markedly better antioxidants than melatonin. In comparison, other effects of tested compounds were very similar. It can be concluded that antioxidant capacity of tested compounds is a major advantage in the early stages of inflammation. Since plasma concentrations of N-acetylserotonin and melatonin are lower than serotonin, it can be deduced that serotonin plays a key role in modulation of inflammation and the regulatory functions of immune cells, while also protecting cells against oxidative stress.
ER -
VAŠÍČEK, Ondřej, Antonín LOJEK a Milan ČÍŽ. Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation. \textit{Journal of Physiology and Biochemistry}. Dordrecht: Springer, 2020, roč.~76, č.~1, s.~49-60. ISSN~1138-7548. Dostupné z: https://dx.doi.org/10.1007/s13105-019-00714-3.
|
