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@article{1705176, author = {Paukovčeková, Silvia and Valík, Dalibor and Štěrba, Jaroslav and Veselská, Renata}, article_location = {Basel}, article_number = {18}, doi = {http://dx.doi.org/10.3390/ijms21186591}, keywords = {osteosarcoma; calcitriol; calcidiol; all-transretinoic acid; vitamin D receptor; retinoic acid receptor alpha}, language = {eng}, issn = {1422-0067}, journal = {International Journal of Molecular Sciences}, title = {Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-Trans Retinoic Acid in Relation to Vitamin D Receptor and Retinoic Acid Receptor alpha Expression in Osteosarcoma Cell Lines}, url = {https://doi.org/10.3390/ijms21186591}, volume = {21}, year = {2020} }
TY - JOUR ID - 1705176 AU - Paukovčeková, Silvia - Valík, Dalibor - Štěrba, Jaroslav - Veselská, Renata PY - 2020 TI - Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-Trans Retinoic Acid in Relation to Vitamin D Receptor and Retinoic Acid Receptor alpha Expression in Osteosarcoma Cell Lines JF - International Journal of Molecular Sciences VL - 21 IS - 18 SP - 1-19 EP - 1-19 PB - MDPI SN - 14220067 KW - osteosarcoma KW - calcitriol KW - calcidiol KW - all-transretinoic acid KW - vitamin D receptor KW - retinoic acid receptor alpha UR - https://doi.org/10.3390/ijms21186591 L2 - https://doi.org/10.3390/ijms21186591 N2 - The main objective of this study was to analyze changes in the antiproliferative effect of vitamin D3, in the form of calcitriol and calcidiol, via its combined application with all-transretinoic acid (ATRA) in osteosarcoma cell lines. The response to treatment with calcitriol and calcidiol alone was specific for each cell line. Nevertheless, we observed an enhanced effect of combined treatment with ATRA and calcitriol in the majority of the cell lines. Although the levels of respective nuclear receptors did not correlate with the sensitivity of cells to these drugs, vitamin D receptor (VDR) upregulation induced by ATRA was found in cell lines that were the most sensitive to the combined treatment. In addition, all these cell lines showed high endogenous levels of retinoic acid receptor alpha (RAR alpha). Our study confirmed that the combination of calcitriol and ATRA can achieve enhanced antiproliferative effects in human osteosarcoma cell lines in vitro. Moreover, we provide the first evidence that ATRA is able to upregulate VDR expression in human osteosarcoma cells. According to our results, the endogenous levels of RAR alpha and VDR could be used as a predictor of possible synergy between ATRA and calcitriol in osteosarcoma cells. ER -
PAUKOVČEKOVÁ, Silvia, Dalibor VALÍK, Jaroslav ŠTĚRBA a Renata VESELSKÁ. Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-Trans Retinoic Acid in Relation to Vitamin D Receptor and Retinoic Acid Receptor alpha Expression in Osteosarcoma Cell Lines. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, roč.~21, č.~18, s.~1-19. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms21186591.
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